Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells.

Autor:	Ferro F; Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy., Azzolin F; Department of Medical, Surgery and Health Sciences, University of Trieste, 34125 Trieste, Italy., Spelat R; Neurobiology Sector, International School for Advanced Studies (SISSA), 34136 Trieste, Italy., Bevilacqua L; Department of Medical, Surgery and Health Sciences, University of Trieste, 34125 Trieste, Italy., Maglione M; Department of Medical, Surgery and Health Sciences, University of Trieste, 34125 Trieste, Italy.
Jazyk:	angličtina
Zdroj:	Journal of functional biomaterials [J Funct Biomater] 2022 Oct 27; Vol. 13 (4). Date of Electronic Publication: 2022 Oct 27.
DOI:	10.3390/jfb13040206
Abstrakt:	Background: Although the influence of titanium implants' micro-surface properties on titanium discs has been extensively investigated, the research has not taken into consideration their whole-body effect, which may be considered possible using a combinatorial approach. Methods: Five titanium dental implants with a similar moderate roughness and different surface textures were thoroughly characterized. The cell adhesion and proliferation were assessed after adipose-tissue-derived stem cells (ADSCs) were seeded on whole-body implants. The implants' inductive properties were assessed by evaluating the osteoblastic gene expression. Results: The surface micro-topography was analyzed, showing that hydroxyapatite (HA)-blasted and bland acid etching implants had the highest roughness and a lower number of surface particles. Cell adhesion was observed after 24 h on all the implants, with the highest score registered for the HA-blasted and bland acid etching implants. Cell proliferation was observed only on the laser-treated and double-acid-etched surfaces. The ADSCs expressed collagen type I, osteonectin, and alkaline phosphatase on all the implant surfaces, with high levels on the HA-treated surfaces, which also triggered osteocalcin expression on day seven. Conclusions: The findings of this study show that the morphology and treatment of whole titanium dental implants, primarily HA-treated and bland acid etching implants, impact the adherence and activity of ADSCs in osteogenic differentiation in the absence of specific osteo-inductive signals.
Databáze:	MEDLINE
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