| Autor: |
Gonçalves MP; Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz, 54538, Fitopatologia e Nematologia, Piracicaba, São Paulo, Brazil; manoel.penachio@usp.br., Simões ALT; Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz, 54538, Fitopatologia e Nematologia, Piracicaba, São Paulo, Brazil; ana.laura.simoes@usp.br., Boufleur TR; USP ESALQ, 54538, Plant Pathology and Nemathology, Av. Pádua Dias, 11, Piracicaba, São Paulo, Brazil, 13418-900; thaisboufleur@usp.br., Santos RFD; Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz, 54538, Fitopatologia e Nematologia, Piracicaba, São Paulo, Brazil; ricardofelicianodossantos@gmail.com., Baroncelli R; Universidad de Salamanca, Departamento de Microbiología y Genética, Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Campus de Villamayor, Calle Del Duero, 12, Villamayor, Salamanca, Spain, 37185; riccardobaroncelli@gmail.com., Amorim L; Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz, 54538, Fitopatologia e Nematologia, Piracicaba, São Paulo, Brazil; lilian.amorim@usp.br. |
| Abstrakt: |
Leaf rust caused by Cerotelium fici (Cast.) Arth. is the main disease affecting Moraceae family plants, such as Ficus and Morus species (Galleti and Rezende 2016; Srikantaswamy et al. 2006). In August 2020, rust symptoms were observed in 100% of mulberry ( Morus nigra L.) trees in an experimental orchard (Piracicaba, SP, Brazil; 22°42'28"S, 47°37'42"W). Mulberry leaves with high rust severity became yellowish and fell-off prematurely. Pustules were light brown with yellowish halo and presented mean size of 0.9 mm 2 . Uredinial paraphyses ( n = 50) measured 42.2 ± 0.67 µm long with wall uniformly ca 0.6-1.1 μm thick. Urediniospores were brownish, echinulate, globoid to broadly ellipsoid, and measured 27.1 ± 0.29 × 21.0 ± 0.27 µm with a wall thickness of 0.6 ± 0.01 µm ( n = 100). The morphology of the urediniospores observed in this study was similar to that reported in the literature for C. fici on Morus alba and Ficus spp. (Gupta et al. 1994; McKenzie 1986; Hennen et al. 2005). We used a low-coverage genome-skimming approach to retrieve genetic information of the rRNA cluster and the mtDNA. Genomic DNA was extracted from 3-4 mg of stored urediniospores at -80 °C, macerated in liquid nitrogen, using a modified cetyl trimethylammonium bromide extraction procedure (Lo Piccolo et al. 2012), and sequenced with 150-bp paired-end reads on Illumina NovaSeq 6000 System. Raw data, (45,761,957 X 2 reads) were assembled with SPAdes v3.15.1 (Bankevich et al., 2012) and the output used to create a custom BLAST database. Loci used for the phylogenetic analyses were identified by BLASTn using, as a query, sequences of C. fici from Ficus sp. from Australia publicly available: Accession No. MH047210.1 for the rRNA and MW036502.1 for COX3. The retrieved sequences were deposited in GenBank under accession numbers OM296992 and OP797407 for the partial rRNA cluster and COX3, respectively. The Bayesian inference phylogenetic analysis of the three concatenate loci (18S, 28S, and COX3) revealed that the isolate obtained in this study (MN1) was clustered in a well-supported clade with C. fici type species. Pathogenicity tests were conducted using mulberry potted plants under greenhouse conditions (25 ± 5 °C). The urediniospores suspension (5 × 10 4 urediniospores ml -1 ) with 0.05% Tween 20 was sprayed with an airbrush on fully expanded leaves until run-off. As a control, mulberry plants were sprayed with distilled water and kept under the same conditions. Inoculated and mock-inoculated plants were kept in a dark moist chamber at 23 °C (± 2 °C) for 24 h. After this period, plants were moved to the greenhouse. The experimental design was completely randomized with five replicates, each replicate consisted of one potted plant and the experiment was performed twice. At 12 days post-inoculation, all inoculated plants showed rust symptoms identical to those observed in the field, whereas control plants had no symptoms. The first symptoms were small pustules on the abaxial surface of fully expanded leaves. Small chlorotic lesions were observed on the adaxial leaf surface, which evolved into necrotic lesions. The pathogen was re-inoculated into potted plants, where it was maintained through monthly inoculations. To our knowledge, this is the first report of mulberry rust on M. nigra in Brazil. As mulberry leaves are the only natural food for silkworm ( Bombyx mori L.), rust poses a significant threat to the sericulture industry because the disease can decrease production and quality of mulberry foliage. |
