Follicle cell contact maintains main body axis polarity in the Drosophila melanogaster oocyte.

Autor:	Milas A; Instituto Gulbenkian de Ciência, Fundação Calouste Gulbenkian, Rua da Quinta Grande, Portugal., de-Carvalho J; Instituto Gulbenkian de Ciência, Fundação Calouste Gulbenkian, Rua da Quinta Grande, Portugal., Telley IA; Instituto Gulbenkian de Ciência, Fundação Calouste Gulbenkian, Rua da Quinta Grande, Portugal.
Jazyk:	angličtina
Zdroj:	The Journal of cell biology [J Cell Biol] 2023 Feb 06; Vol. 222 (2). Date of Electronic Publication: 2022 Nov 21.
DOI:	10.1083/jcb.202209052
Abstrakt:	In Drosophila melanogaster, the anterior-posterior body axis is maternally established and governed by differential localization of partitioning defective (Par) proteins within the oocyte. At mid-oogenesis, Par-1 accumulates at the oocyte posterior end, while Par-3/Bazooka is excluded there but maintains its localization along the remaining oocyte cortex. Past studies have proposed the need for somatic cells at the posterior end to initiate oocyte polarization by providing a trigger signal. To date, neither the molecular identity nor the nature of the signal is known. Here, we provide evidence that mechanical contact of posterior follicle cells (PFCs) with the oocyte cortex causes the posterior exclusion of Bazooka and maintains oocyte polarity. We show that Bazooka prematurely accumulates exclusively where posterior follicle cells have been mechanically detached or ablated. Furthermore, we provide evidence that PFC contact maintains Par-1 and oskar mRNA localization and microtubule cytoskeleton polarity in the oocyte. Our observations suggest that cell-cell contact mechanics modulates Par protein binding sites at the oocyte cortex. (© 2022 Milas et al.)
Databáze:	MEDLINE
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