Autor: |
Pattison JR, Jackson CM, Hiscock JA, Cradock-Watson JE, Ridehalgh MK |
Jazyk: |
angličtina |
Zdroj: |
Journal of medical microbiology [J Med Microbiol] 1978 Nov; Vol. 11 (4), pp. 411-8. |
DOI: |
10.1099/00222615-11-4-411 |
Abstrakt: |
Serum specimens from 14 infants with congenital rubella were examined for specific IgM antibody by six different methods. IgM-containing fractions were separated either by sucrose density-gradient centrifugation or by gel filtration through Sephadex G-200, and were then tested by the indirect immunofluorescence technique and by the haemagglutination-inhibition (HI) test (long-and short-incubation methods). Immunofluorescence staining of density-gradient fractions detected specific IgM in all 14 infants. The HI test (long method), applied to density-gradient fractions, was almost as sensitive, detecting antibody in 13 infants; the short method was less sensitive. The gel-filtration technique proved to be generally less satisfactory than sucrose density-gradient centrifugation. Evidence was obtained for the occurrence of as yet unclassified non-specific inhibitors in the serum of some infants. These inhibitors were deposited with the IgM on sucrose-density gradients and they could have been mistaken for rubella-specific IgM antibody, particularly in the HI test (long method). |
Databáze: |
MEDLINE |
Externí odkaz: |
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