Similar bacterial signatures in the gut microbiota of type 1 and type 2 diabetes patients and its association with G protein-coupled receptor 41 and 43 gene expression.

Autor: Demirci M; School of Medicine, Department of Medical Microbiology, Kirklareli University, Kirklareli, Turkey., Taner Z; Altinbas School of Medicine, Department of Medical Microbiology, Altinbas University, Istanbul, Turkey., Keskin FE; School of Medicine, Department of Endocrinology, Demiroğlu Bilim University, Istanbul, Turkey., Ozyazar M; Cerrahpasa School of Medicine, Department of Endocrinology, Istanbul University-Cerrahpasa, Istanbul, Turkey., Kiraz N; School of Medicine, Department of Medical Microbiology, Namık Kemal University, Tekirdag, Turkey., Kocazeybek BS; Cerrahpasa School of Medicine, Department of Medical Microbiology, Istanbul University-Cerrahpasa, Istanbul, Turkey., Tokman HB; Cerrahpasa School of Medicine, Department of Medical Microbiology, Istanbul University-Cerrahpasa, Istanbul, Turkey.
Jazyk: angličtina
Zdroj: Journal of diabetes and metabolic disorders [J Diabetes Metab Disord] 2022 Jun 30; Vol. 21 (2), pp. 1359-1368. Date of Electronic Publication: 2022 Jun 30 (Print Publication: 2022).
DOI: 10.1007/s40200-022-01068-2
Abstrakt: Purpose: There are conflicting reports regarding the abundance of short-chain fatty acids producing bacteria in the gut microbiota in patients with type 1 and type 2 diabetes. We aimed to determine the amount of Akkermansia muciniphila, Anaerobutyricum hallii, Bifidobacterium adolescentis, Bifidobacterium longum, Collinsella aerofaciens, Faecalibacterium prausnitzii, Lacticaseibacillus rhamnosus , and Parabacteroides distasonis in the gut microbiota in patients with type1 and type2 diabetes, compared with the healthy controls and analyze the correlation between the gene expression levels of two s hort-chain fatty acids receptors GPR41 and GPR43.
Methods: Forty type 1, 40 type 2 stool and blood samples of diabetes patients, and 40 healthy control samples were studied. DNA and RNA were extracted, and bacteria were detected using a Microbial DNA qPCR Assay kit. Gene expressions were detected with GPR41 and GPR43 primers via in-house qPCR.
Results: Compared with healthy controls, B.longum and F.prausnitzii abundance were significantly decreased in patients with type1 and type2 diabetes, A.hallii abundance was increased in patients with type1 and decreased in type2 diabetes contrarily A.muciniphila abundance was decreased in patients with type1 and increased in type2 diabetes. GPR43 g ene expression was upregulated in both patients group, however GPR41 was upregulated only in patients with type2 diabetes.
Conclusions: Elevated B. longum and F. prausnitzii abundances were detected in the gut microbiota of patients with type1 and type2 diabetes and compared with healthy controls. B. longum and F.prausnitzii abundances were also correlated with the GPR43 gene expression level in type1 diabetes patients. Extensive studies determining bacteria producing short-chain fatty acids in gut microbiota, and their contribution in the pathogenesis of diabetes, are needed to understand better the mechanism of these diseases.
Competing Interests: Conflict of interestAll authors state that we don’t have any potential conflict of interest including any financial activities, additional affiliations, personal or other relationships with other people or organizations that could influence, or be perceived to influence, their work, such as employment, consultancies, stock ownership, honoraria, patent applications/registrations, grants or other funding.
(© The Author(s), under exclusive licence to Tehran University of Medical Sciences 2022.)
Databáze: MEDLINE
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