Surface Engineering of Auxetic Scaffolds for Neural and Vascular Differentiation from Human Pluripotent Stem Cells.

Autor: Chen X; Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer St. 131, Tallahassee, FL, 32310, USA.; High-Performance Materials Institute, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, 32310, USA., Liu C; Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer St. 131, Tallahassee, FL, 32310, USA., Wadsworth M; High-Performance Materials Institute, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, 32310, USA., Zeng EZ; Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer St. 131, Tallahassee, FL, 32310, USA., Driscoll T; Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer St. 131, Tallahassee, FL, 32310, USA., Zeng C; High-Performance Materials Institute, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, 32310, USA.; Department of Industrial and Manufacturing Engineering, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL, 32310, USA., Li Y; Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer St. 131, Tallahassee, FL, 32310, USA.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2023 Jan; Vol. 12 (6), pp. e2202511. Date of Electronic Publication: 2022 Dec 04.
DOI: 10.1002/adhm.202202511
Abstrakt: Auxetic materials are the materials that can display negative Poisson's ratio that describes the degree to which a material contracts (or expands) transversally when axially strained. Human stem cells sense the mechanical properties of the microenvironment, including material surface properties, stiffness, and Poisson's ratio. In this study, six different auxetic polyurethane (PU) foams with different elastic modulus (0.7-1.8 kPa) and Poisson's ratio (-0.1 to -0.5) are used to investigate lineage specification of human induced pluripotent stem cells (hiPSCs). The surfaces of the foams are modified with chitosan or heparin to enhance the adhesion and proliferation of hiPSCs. Then, the vascular and neural differentiation of hiPSCs are investigated on different foams with distinct elastic modulus and Poisson's ratio. With different auxetic foams, cells show differential adherent density and differentiation capacity. Chitosan and heparin surface functionalization promote the hindbrain and hippocampal markers, but not forebrain markers during neural patterning of hiPSCs. Properly surface engineered auxetic scaffolds can also promote vascular differentiation of hiPSCs. This study represents a versatile and multifunctional scaffold fabrication approach and can lead to a suitable system for establishing hiPSC culture models in applications of neurovascular disease modeling and drug screening.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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