MRI Evaluation of Gene Therapy in the Canine Model of Duchenne Muscular Dystrophy.

Autor:	Zalcman AR; Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, The University of Missouri, Columbia, MO, USA.; VetCT, Orlando, FL, USA., Hakim CH; Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri, Columbia, MO, USA., Lattimer JC; Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, The University of Missouri, Columbia, MO, USA., Holland JR; Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, The University of Missouri, Columbia, MO, USA., Dodam JR; Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, The University of Missouri, Columbia, MO, USA., Duan D; Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri, Columbia, MO, USA. duand@missouri.edu.; Department of Biomedical, Biological & Chemical Engineering, College of Engineering, The University of Missouri, Columbia, MO, USA. duand@missouri.edu.; Department of Neurology, School of Medicine, The University of Missouri, Columbia, MO, USA. duand@missouri.edu.; Department of Biomedical Sciences, College of Veterinary Medicine, The University of Missouri, Columbia, MO, USA. duand@missouri.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2587, pp. 339-352.
DOI:	10.1007/978-1-0716-2772-3_17
Abstrakt:	Magnetic resonance imaging (MRI) is a well-established and widely used technique to characterize and quantify skeletal and cardiac muscle changes in Duchenne muscular dystrophy (DMD). Recently, MRI has been explored to study disease progression and response to gene therapy in the canine DMD model. Using traditional sequences, delayed gadolinium enhancement, novel sequences, and spectroscopy, investigators have begun to (i) establish the baseline MRI characteristics of the muscles in normal and affected dogs and (ii) evaluate gene therapy outcomes in treated dogs. As a noninvasive assay, MRI offers an excellent opportunity to study longitudinal muscle changes in long-term gene therapy studies in the canine model. In this chapter, we outline the MRI method used to study DMD in the canine model. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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