Comparative diagnostic accuracy between simplified and original flow cytometric gating strategies for peripheral blood neutrophil myeloperoxidase expression in ruling out myelodysplastic syndromes.

Autor: Raskovalova T; Institute for Advanced Biosciences, INSERM U1209, CNRS UMR 5309, Université Grenoble Alpes, Grenoble, France.; Laboratoire d'Immunologie, Grenoble University Hospital, Grenoble, France., Scheffen L; Institute for Advanced Biosciences, INSERM U1209, CNRS UMR 5309, Université Grenoble Alpes, Grenoble, France., Jacob MC; Institute for Advanced Biosciences, INSERM U1209, CNRS UMR 5309, Université Grenoble Alpes, Grenoble, France.; Laboratoire d'Immunologie, Grenoble University Hospital, Grenoble, France., Vettier C; Laboratoire d'Hématologie Biologique, Grenoble University Hospital, Grenoble, France., Bulabois B; Laboratoire d'Hématologie Biologique, Grenoble University Hospital, Grenoble, France., Szymanski G; Laboratoire d'Hématologie Biologique, Grenoble University Hospital, Grenoble, France., Chevalier S; Laboratoire d'Hématologie Biologique, Grenoble University Hospital, Grenoble, France., Gonnet N; CIC 1406, INSERM, Université Grenoble Alpes, Grenoble University Hospital, Grenoble, France., Park S; Institute for Advanced Biosciences, INSERM U1209, CNRS UMR 5309, Université Grenoble Alpes, Grenoble, France.; Clinique Universitaire d'Hématologie, Grenoble University Hospital, Grenoble, France., Labarère J; Clinical Epidemiology Unit, Grenoble University Hospital, Grenoble, France.; TIMC-IMAG, UMR 5525, CNRS, Université Grenoble Alpes, Grenoble, France.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Nov 18; Vol. 17 (11), pp. e0276095. Date of Electronic Publication: 2022 Nov 18 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0276095
Abstrakt: Background: Flow cytometric analysis of peripheral blood neutrophil myeloperoxidase expression is accurate in ruling out myelodyplastic syndromes (MDS) but might not be suitable for implementation in busy clinical laboratories. We aimed to simplify the original gating strategy and examine its accuracy.
Methods: Using the individual data from 62 consecutive participants enrolled in a prospective validation study, we assessed the agreement in intra-individual robust coefficient of variation (RCV) of peripheral blood neutrophil myeloperoxidase expression and compared diagnostic accuracy between the simplified and original gating strategies.
Results: Cytomorphological evaluation of bone marrow aspirate confirmed MDS in 23 patients (prevalence, 37%), unconfirmed MDS in 32 patients (52%), and was uninterpretable in 7 patients (11%). Median intra-individual RCV for simplified and original gating strategies were 30.7% (range, 24.7-54.4) and 30.6% (range, 24.7-54.1), with intra-class correlation coefficient quantifying absolute agreement equal to 1.00 (95% confidence interval [CI], 0.99 to 1.00). The areas under the receiver operating characteristic (ROC) curves were 0.93 (95% CI, 0.82-0.98) and 0.92 (95% CI, 0.82-0.98), respectively (P = .32). Using simplified or original gating strategy, intra-individual RCV values lower than a pre-specified threshold of 30.0% ruled out MDS for 35% (19 of 55) patients, with both sensitivity and negative predictive value estimates of 100%.
Conclusions: The simplified gating strategy performs as well as the original one for ruling out MDS and has the potential to save time and reduce resource utilization. Yet, prospective validation of the simplified gating strategy is warranted before its adoption in routine.
Trial Registration: ClinicalTrials.gov Identifier: NCT03363399 (First posted on December 6, 2017).
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2022 Raskovalova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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