Three-Dimensional Biohybrid StarPEG-Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer's Disease Pathology.

Autor:	Siddiqui T; German Center for Neurodegenerative Diseases (DZNE) Dresden, Helmholtz Association, Dresden, Germany., Celikkaya H; German Center for Neurodegenerative Diseases (DZNE) Dresden, Helmholtz Association, Dresden, Germany., Atasavum ZT; German Center for Neurodegenerative Diseases (DZNE) Dresden, Helmholtz Association, Dresden, Germany.; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Dresden, Germany., Popova S; German Center for Neurodegenerative Diseases (DZNE) Dresden, Helmholtz Association, Dresden, Germany.; Neuron-D GmbH, Dresden, Germany., Freudenberg U; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Dresden, Germany., Werner C; Leibniz-Institut für Polymerforschung Dresden e.V., Max Bergmann Center of Biomaterials Dresden, Dresden, Germany., Kizil C; German Center for Neurodegenerative Diseases (DZNE) Dresden, Helmholtz Association, Dresden, Germany. ck2893@cumc.columbia.edu.; Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University Irving Medical Center, New York, NY, USA. ck2893@cumc.columbia.edu.; Department of Neurology, Columbia University Irving Medical Center, New York, NY, USA. ck2893@cumc.columbia.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2561, pp. 159-170.
DOI:	10.1007/978-1-0716-2655-9_8
Abstrakt:	In this chapter, we present the methodology currently used in our laboratory to generate a starPEG-MMP (starPEG)- and heparin maleimide HM06 (heparin)-based 3D cell culture system, in a hydrogel, that can be used to study human neuronal development and Alzheimer's disease (AD) pathology. A 3D cell culture system can mimic the in vivo cellular environment better than a 2D format, in which these cells exhibit neural network formation, electrophysiological activity, tissue-specific extracellular matrix (ECM) deposition, and neurotransmitter responsiveness. When treated with amyloid beta-42 (Aβ42) peptides, this system recapitulates many of the pathological effects of AD, including reduced neural stem cell proliferation, impaired neuronal network formation, dystrophic axonal ends, synaptic loss, failure to deposit ECM, elevated tau hyperphosphorylation, and formation of neurofibrillary tangles. Culturing human primary cortical astrocyte (pHA)- or induced pluripotent stem cell (iPSC)-derived human neural stem cells in this biohybrid hydrogel system has led to the discovery of novel regulatory pathways underlying neurodegenerative pathology in different phases of AD. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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