Illuminati: a form of gene expression plasticity in Drosophila neural stem cells.

Autor:	Goupil A; Institut Curie, Paris Science et Lettres Research University, Centre National de la Recherche Scientifique, Unité Mixte de Recherche UMR144, Biology of Centrosomes and Genetic Instability Laboratory, 75005 Paris, France., Heinen JP; Institute for Research in Biomedicine (IRB Barcelona), Cell Division Laboratory, Cancer Science Programme, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain., Salame R; Institut Curie, Paris Science et Lettres Research University, Centre National de la Recherche Scientifique, Unité Mixte de Recherche UMR144, Biology of Centrosomes and Genetic Instability Laboratory, 75005 Paris, France., Rossi F; Institute for Research in Biomedicine (IRB Barcelona), Cell Division Laboratory, Cancer Science Programme, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain., Reina J; Institute for Research in Biomedicine (IRB Barcelona), Cell Division Laboratory, Cancer Science Programme, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain., Pennetier C; Institut Curie, Paris Science et Lettres Research University, Centre National de la Recherche Scientifique, Unité Mixte de Recherche UMR144, Biology of Centrosomes and Genetic Instability Laboratory, 75005 Paris, France., Simon A; Institut Curie, Paris Science et Lettres Research University, Centre National de la Recherche Scientifique, Unité Mixte de Recherche UMR144, Biology of Centrosomes and Genetic Instability Laboratory, 75005 Paris, France., Skorski P; Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, Stem Cells and Tissue Homeostasis Group, 75005 Paris, France., Louzao A; Institute for Research in Biomedicine (IRB Barcelona), Cell Division Laboratory, Cancer Science Programme, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain., Bardin AJ; Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, Stem Cells and Tissue Homeostasis Group, 75005 Paris, France., Basto R; Institut Curie, Paris Science et Lettres Research University, Centre National de la Recherche Scientifique, Unité Mixte de Recherche UMR144, Biology of Centrosomes and Genetic Instability Laboratory, 75005 Paris, France., Gonzalez C; Institute for Research in Biomedicine (IRB Barcelona), Cell Division Laboratory, Cancer Science Programme, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain.; Catalan Institution for Research and Advanced Studies (ICREA), 08010 Barcelona, Spain.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2022 Nov 15; Vol. 149 (22). Date of Electronic Publication: 2022 Nov 18.
DOI:	10.1242/dev.200808
Abstrakt:	While testing for genome instability in Drosophila as reported by unscheduled upregulation of UAS-GFP in cells that co-express GAL80 and GAL4, we noticed that, as expected, background levels were low in most developing tissues. However, GFP-positive clones were frequent in the larval brain. Most of these clones originated from central brain neural stem cells. Using imaging-based approaches and genome sequencing, we show that these unscheduled clones do not result from chromosome loss or mutations in GAL80. We have named this phenomenon 'Illuminati'. Illuminati is strongly enhanced in brat tumors and is also sensitive to environmental conditions such as food content and temperature. Illuminati is suppressed by Su(var)2-10, but it is not significantly affected by several modifiers of position effect variegation or Gal4::UAS variegation. We conclude that Illuminati identifies a previously unknown type of functional instability that may have important implications in development and disease. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2022. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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