Sequencing of Camelina neglecta, a diploid progenitor of the hexaploid oilseed Camelina sativa.

Autor: Chaudhary R; Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.; Global Institute for Food Security, Saskatoon, SK, Canada., Koh CS; Global Institute for Food Security, Saskatoon, SK, Canada., Perumal S; Global Institute for Food Security, Saskatoon, SK, Canada., Jin L; Department of Computer Science, University of Saskatchewan, Saskatoon, SK, Canada., Higgins EE; Agriculture and Agri-Food Canada, Saskatoon, SK, Canada., Kagale S; National Research Council Canada, Saskatoon, SK, Canada., Smith MA; Agriculture and Agri-Food Canada, Saskatoon, SK, Canada., Sharpe AG; Global Institute for Food Security, Saskatoon, SK, Canada., Parkin IAP; Agriculture and Agri-Food Canada, Saskatoon, SK, Canada.
Jazyk: angličtina
Zdroj: Plant biotechnology journal [Plant Biotechnol J] 2023 Mar; Vol. 21 (3), pp. 521-535. Date of Electronic Publication: 2022 Dec 12.
DOI: 10.1111/pbi.13968
Abstrakt: Camelina neglecta is a diploid species from the genus Camelina, which includes the versatile oilseed Camelina sativa. These species are closely related to Arabidopsis thaliana and the economically important Brassica crop species, making this genus a useful platform to dissect traits of agronomic importance while providing a tool to study the evolution of polyploids. A highly contiguous chromosome-level genome sequence of C. neglecta with an N50 size of 29.1 Mb was generated utilizing Pacific Biosciences (PacBio, Menlo Park, CA) long-read sequencing followed by chromosome conformation phasing. Comparison of the genome with that of C. sativa shows remarkable coincidence with subgenome 1 of the hexaploid, with only one major chromosomal rearrangement separating the two. Synonymous substitution rate analysis of the predicted 34 061 genes suggested subgenome 1 of C. sativa directly descended from C. neglecta around 1.2 mya. Higher functional divergence of genes in the hexaploid as evidenced by the greater number of unique orthogroups, and differential composition of resistant gene analogs, might suggest an immediate adaptation strategy after genome merger. The absence of genome bias in gene fractionation among the subgenomes of C. sativa in comparison with C. neglecta, and the complete lack of fractionation of meiosis-specific genes attests to the neopolyploid status of C. sativa. The assembled genome will provide a tool to further study genome evolution processes in the Camelina genus and potentially allow for the identification and exploitation of novel variation for Camelina crop improvement.
(© 2022 National Research Council Canada and The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. Reproduced with the permission of the Minister of Innovation, Science, and Economic Development and Agriculture and Agri-Food Canada.)
Databáze: MEDLINE
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