Cerebrospinal fluid analysis in emergency patients with suspected infection of the central nervous system.

Autor: Völk S; Department of Neurology, University Hospital, LMU, Munich, Germany., Dobler F; Department of Neurology, University Hospital, LMU, Munich, Germany., Koedel U; Department of Neurology, University Hospital, LMU, Munich, Germany., Pfister HW; Department of Neurology, University Hospital, LMU, Munich, Germany., Bruegel M; Institute of Laboratory Medicine, University Hospital, LMU, Munich, Germany., Schubert S; Max von Pettenkofer-Institute, Faculty of Medicine, LMU, Munich, Germany., Klein M; Department of Neurology, University Hospital, LMU, Munich, Germany.; Emergency Department, University Hospital, LMU, Munich, Germany.
Jazyk: angličtina
Zdroj: European journal of neurology [Eur J Neurol] 2023 Mar; Vol. 30 (3), pp. 702-709. Date of Electronic Publication: 2022 Nov 29.
DOI: 10.1111/ene.15638
Abstrakt: Background and Purpose: Meningitis and encephalitis are potentially life-threatening diseases that require fast and accurate diagnostics and therapy. The value of polymerase chain reaction (PCR) multiplex testing in clinical practice is still a matter of debate. This study aims to evaluate its benefits and limitations in emergency patients.
Methods: We assessed the value of a meningoencephalitis PCR array in the clinical routine of an emergency department.
Results: Of 1578 emergency patients who received a lumbar puncture, 43% received it for a clinically suspected central nervous system (CNS) infection. After initial workup for cerebrospinal fluid (CSF) cell count, protein and glucose, a CNS infection was still considered likely in 307 patients. In these patients, further microbiologic workup was performed. A total of 230 samples were examined by PCR and a pathogen was detected in 66 of these samples. In the case of a positive microbiologic result, a comparison between PCR array and standard method was available for 59 samples, which demonstrated an overcall agreement of 80% (n = 47/59). Of interest, exclusively array-positive results were observed for patients with meningitis found to be positive for Streptococcus pneumoniae; four out of five patients had been treated with antibiotics before the lumbar puncture. In samples with normal CSF cell count only two positive array results were obtained, both for human herpesvirus 6, and these were not clinically relevant.
Conclusion: Our data suggest that the array substantially contributes to a detection of pathogens in patients with suspected CNS infection and seems of particular interest in patients with acute bacterial meningitis under empiric antibiotic treatment. In CSF samples with normal cell count, it might be dispensable.
(© 2022 The Authors. European Journal of Neurology published by John Wiley & Sons Ltd on behalf of European Academy of Neurology.)
Databáze: MEDLINE
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