Lactoferrin and the development of salivary stones: a pilot study.

Autor: Kraaij S; Department of Oral and Maxillofacial Surgery/Oral Pathology, Amsterdam University Medical Centers, Location VUmc, and Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. saskiakraay@hotmail.com.; Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. saskiakraay@hotmail.com.; Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Room 12N-37, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands. saskiakraay@hotmail.com., de Visscher JGAM; Department of Oral and Maxillofacial Surgery/Oral Pathology, Amsterdam University Medical Centers, Location VUmc, and Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands., Apperloo RC; Department of Oral and Maxillofacial Surgery, Amsterdam University Medical Centers, Location AMC, Amsterdam, The Netherlands., Nazmi K; Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands., Bikker FJ; Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands., Brand HS; Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands.
Jazyk: angličtina
Zdroj: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine [Biometals] 2023 Jun; Vol. 36 (3), pp. 657-665. Date of Electronic Publication: 2022 Nov 17.
DOI: 10.1007/s10534-022-00465-7
Abstrakt: Salivary stones (sialoliths) are calcified structures located in the ductal system of the major salivary glands. Their exact cause is not clear but in general they are characterized by concentric inorganic (hydroxyapatite) layers. The formation is a slow intermittent process which may result in enlargement of the sialolith causing obstruction of saliva secretion resulting in mealtime related pain and swelling of the affected salivary gland. Various studies reported the presence of organic material such as proteins and lipids in the core of sialoliths. In the present study the protein composition of twenty submandibular sialoliths was analyzed. It was found that proteins contributed on average 5% to the dry weight of submandibular stones whereby small salivary stones contained more extractable proteins than large salivary stones. Using a combination of SDS-PAGE gel electrophoresis and Western blotting, we identified α-amylase (in all stones; 100%), lysozyme (95%), lactoferrin (85%), secretory-IgA (75%), MUC7 (60%), complement C4 (60%) and C-reactive protein (35%). The presence, and the combinations, of lactoferrin, lysozyme, s-IgA and α-amylase in sialoliths was confirmed by ELISA. The gradually increasing size of a sialolith might provoke a local inflammatory response in the duct of the submandibular gland whereby the relatively low concentrations of lactoferrin and lysozyme may originate from neutrophils. The interaction of lactoferrin with s-IgA could contribute to the accumulation of lactoferrin in sialoliths. In summary, these results suggest a new pathophysiological role for lactoferrin, in the formation of sialoliths.
(© 2022. The Author(s).)
Databáze: MEDLINE
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