Folic acid incorporated nitrogen-doped carbon dots as a turn-on fluorescence probe for homocysteine detection.

Autor: Anju SM; Department of Chemistry, School of Physical and Mathematical Science, University of Kerala, Thiruvananthapuram, Kerala, India., Aswathy AO; Department of Chemistry, School of Physical and Mathematical Science, University of Kerala, Thiruvananthapuram, Kerala, India., Varghese S; Department of Chemistry, School of Physical and Mathematical Science, University of Kerala, Thiruvananthapuram, Kerala, India., Abraham MK; Department of Chemistry, School of Physical and Mathematical Science, University of Kerala, Thiruvananthapuram, Kerala, India., Lekshmi RS; Department of Chemistry, School of Physical and Mathematical Science, University of Kerala, Thiruvananthapuram, Kerala, India., Ibrahim Shkhair A; Department of Chemistry, School of Physical and Mathematical Science, University of Kerala, Thiruvananthapuram, Kerala, India., Lekha GM; Department of Chemistry, School of Physical and Mathematical Science, University of Kerala, Thiruvananthapuram, Kerala, India., Syamchand SS; Department of Chemistry, University College, Thiruvananthapuram, Kerala, India., George S; Department of Chemistry, School of Physical and Mathematical Science, University of Kerala, Thiruvananthapuram, Kerala, India.
Jazyk: angličtina
Zdroj: Luminescence : the journal of biological and chemical luminescence [Luminescence] 2023 Jan; Vol. 38 (1), pp. 19-27. Date of Electronic Publication: 2022 Dec 05.
DOI: 10.1002/bio.4411
Abstrakt: This study describes the development of a low-cost fluorescence assay for detecting homocysteine (Hcy) without the interference of cysteine and glutathione using carbon quantum dots. Herein nitrogen-doped carbon quantum dots (NCDs) were synthesized from citric acid as the carbon source and urea as the dopant using a one-pot microwave-assisted method. The obtained NCDs were incorporated with folic acid (FA) by the direct ex situ addition method and were used as a fluorescence probe to detect Hcy. The probe exhibited a fluorescence turn-on response with increased Hcy concentration up to 50 μM with a limit of detection of 2.276 μM. The point of care detection of Hcy using the probe was also tested with a paper-based assay strip.
(© 2022 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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