Modification of phosphoinositides by the Shigella effector IpgD during host cell infection.

Autor: Tran Van Nhieu G; Institute for Integrative Biology of the Cell - Centre National de la Recherche Scientifique (CNRS) UMR9198 - Institut National de la Santé et de la Recherche Médicale (Inserm) U1280, Team Calcium Signaling and Microbial Infections, Gif-sur-Yvette, France., Latour-Lambert P; Institut Pasteur, Unité Dynamique des interactions hôtes-pathogènes and Centre National de la Recherche Scientifique (CNRS) UMR3691, Université de Paris Cité, Paris, France., Enninga J; Institut Pasteur, Unité Dynamique des interactions hôtes-pathogènes and Centre National de la Recherche Scientifique (CNRS) UMR3691, Université de Paris Cité, Paris, France.
Jazyk: angličtina
Zdroj: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2022 Oct 27; Vol. 12, pp. 1012533. Date of Electronic Publication: 2022 Oct 27 (Print Publication: 2022).
DOI: 10.3389/fcimb.2022.1012533
Abstrakt: Shigella , the causative agent of bacillary dysentery, subvert cytoskeletal and trafficking processes to invade and replicate in epithelial cells using an arsenal of bacterial effectors translocated through a type III secretion system. Here, we review the various roles of the type III effector IpgD, initially characterized as phosphatidylinositol 4,5 bisphosphate (PI4,5P 2 ) 4-phosphatase. By decreasing PI4,5P 2 levels, IpgD triggers the disassembly of cortical actin filaments required for bacterial invasion and cell migration. PI5P produced by IpgD further stimulates signaling pathways regulating cell survival, macropinosome formation, endosomal trafficking and dampening of immune responses. Recently, IpgD was also found to exhibit phosphotransferase activity leading to PI3,4P 2 synthesis adding a new flavor to this multipotent bacterial enzyme. The substrate of IpgD, PI4,5P 2 is also the main substrate hydrolyzed by endogenous phospholipases C to produce inositoltriphosphate (InsP 3 ), a major Ca 2+ second messenger. Hence, beyond the repertoire of effects associated with the direct diversion of phoshoinositides, IpgD indirectly down-regulates InsP 3 -mediated Ca 2+ release by limiting InsP 3 production. Furthermore, IpgD controls the intracellular lifestyle of Shigella promoting Rab8/11 -dependent recruitment of the exocyst at macropinosomes to remove damaged vacuolar membrane remnants and promote bacterial cytosolic escape. IpgD thus emerges as a key bacterial effector for the remodeling of host cell membranes.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Tran Van Nhieu, Latour-Lambert and Enninga.)
Databáze: MEDLINE
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