A distributable LC-MS/MS method for the measurement of serum thyroglobulin.

Autor: Shi J; Departments of Laboratory Medicine and Pathology, USA., Phipps WS; Departments of Laboratory Medicine and Pathology, USA., Owusu BY; Departments of Laboratory Medicine and Pathology, USA., Henderson CM; Departments of Laboratory Medicine and Pathology, USA., Laha TJ; Departments of Laboratory Medicine and Pathology, USA., Becker JO; Departments of Laboratory Medicine and Pathology, USA., Razavi M; SISCAPA Assay Technologies, Inc, Washington, DC, USA., Emrick MA; Departments of Laboratory Medicine and Pathology, USA., Hoofnagle AN; Departments of Laboratory Medicine and Pathology, USA.; Medicine, University of Washington School of Medicine, Seattle, WA, USA.
Jazyk: angličtina
Zdroj: Journal of mass spectrometry and advances in the clinical lab [J Mass Spectrom Adv Clin Lab] 2022 Sep 19; Vol. 26, pp. 28-33. Date of Electronic Publication: 2022 Sep 19 (Print Publication: 2022).
DOI: 10.1016/j.jmsacl.2022.09.005
Abstrakt: Background: Despite its clear advantages over immunoassay-based testing, the measurement of serum thyroglobulin by mass spectrometry remains limited to a handful of institutions. Slow adoption by clinical laboratories could reflect limited accessibility to existing methods that have sensitivity comparable to modern immunoassays, as well as a lack of tools for calibration and assay harmonization.
Methods: We developed and validated a liquid chromatography-tandem mass spectrometry-based assay for the quantification of serum thyroglobulin. The protocol combined peptide immunoaffinity purification using a commercially available, well-characterized monoclonal antibody and mobile phase modification with dimethylsulfoxide (DMSO) for enhanced sensitivity. To facilitate harmonization with other laboratories, we developed a novel, serum-based 5-point distributable reference material (Husky Ref).
Results: The assay demonstrated a lower limit of quantification of 0.15 ng/mL (<20 %CV). Mobile phase DMSO increased signal intensity of the target peptide at least 3-fold, improving quantification at low concentrations. Calibration traceable to Husky Ref enabled harmonization between laboratories in an interlaboratory study.
Conclusions: Sensitive mass spectrometry-based thyroglobulin measurement can be achieved using a monoclonal antibody during peptide immunoaffinity purification and the addition of mobile phase DMSO. Laboratories interested in deploying this assay can utilize the provided standard operating procedure and freely-available Husky Ref reference material.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2022 THE AUTHORS.)
Databáze: MEDLINE
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