3D visualization of trans-syncytial nanopores provides a pathway for paracellular diffusion across the human placental syncytiotrophoblast.

Autor:	Lewis RM; Faculty of Medicine, Southampton General Hospital, University of Southampton, MP 887, IDS Building, University Road, Southampton SO17 1BJ, UK.; Institute for Life Sciences, University of Southampton, University Road, Southampton SO17 1BJ, UK., Baskaran H; Faculty of Engineering and Physical Sciences, University of Southampton, University Road, Southampton SO17 1BJ, UK., Green J; Faculty of Medicine, Southampton General Hospital, University of Southampton, MP 887, IDS Building, University Road, Southampton SO17 1BJ, UK., Tashev S; Faculty of Medicine, Biomedical Imaging Unit, University of Southampton, University Road, Southampton SO17 1BJ, UK., Palaiologou E; Faculty of Medicine, Southampton General Hospital, University of Southampton, MP 887, IDS Building, University Road, Southampton SO17 1BJ, UK., Lofthouse EM; Faculty of Medicine, Southampton General Hospital, University of Southampton, MP 887, IDS Building, University Road, Southampton SO17 1BJ, UK., Cleal JK; Faculty of Medicine, Southampton General Hospital, University of Southampton, MP 887, IDS Building, University Road, Southampton SO17 1BJ, UK.; Institute for Life Sciences, University of Southampton, University Road, Southampton SO17 1BJ, UK., Page A; Faculty of Medicine, Biomedical Imaging Unit, University of Southampton, University Road, Southampton SO17 1BJ, UK., Chatelet DS; Faculty of Medicine, Biomedical Imaging Unit, University of Southampton, University Road, Southampton SO17 1BJ, UK., Goggin P; Faculty of Medicine, Biomedical Imaging Unit, University of Southampton, University Road, Southampton SO17 1BJ, UK., Sengers BG; Institute for Life Sciences, University of Southampton, University Road, Southampton SO17 1BJ, UK.; Faculty of Engineering and Physical Sciences, University of Southampton, University Road, Southampton SO17 1BJ, UK.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2022 Oct 28; Vol. 25 (12), pp. 105453. Date of Electronic Publication: 2022 Oct 28 (Print Publication: 2022).
DOI:	10.1016/j.isci.2022.105453
Abstrakt:	The placental syncytiotrophoblast, a syncytium without cell-cell junctions, is the primary barrier between the mother and the fetus. Despite no apparent anatomical pathway for paracellular diffusion of solutes across the syncytiotrophoblast, size-dependent paracellular diffusion is observed. Here we report data demonstrating that the syncytiotrophoblast is punctuated by trans -syncytial nanopores (TSNs). These membrane-bound TSNs directly connect the maternal and fetal facing sides of the syncytiotrophoblast, providing a pathway for paracellular diffusion between the mother and fetus. Mathematical modeling of TSN permeability based on their 3D geometry suggests that 10-37 million TSNs per cm 3 of placental tissue could explain experimentally observed placental paracellular diffusion. TSNs may mediate physiological hydrostatic and osmotic pressure homeostasis between the maternal and fetal circulations but also expose the fetus to pharmaceuticals, environmental pollutants, and nanoparticles. Competing Interests: The authors have no competing interests to declare. (© 2022 The Author(s).)
Databáze:	MEDLINE
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