Development and characterization of trans-anethole-containing solid lipid microparticles: antiinflammatory and gastroprotective effects in experimental inflammation.

Autor: da Rocha EMT; Department of Pharmacology and Therapeutics, Bloco K68, State University of Maringá (UEM), Avenue Colombo, 5790, Jd. Universitário, 87020-900, Maringá, Brazil., Bracht L; Department of Biochemistry, State University of Maringá (UEM), Avenue Colombo, 5790, Jd. Universitário, Maringá, PR, 87020-900, Brazil., Gonçalves OH; Post-Graduation Program of Food Technology (PPGTA), Federal University of Technology - Paraná (UTFPR), BR 369, Km 0.5, POBox 271, Campo Mourão, PR, 87301-006, Brazil., Leimann FV; Post-Graduation Program of Food Technology (PPGTA), Federal University of Technology - Paraná (UTFPR), BR 369, Km 0.5, POBox 271, Campo Mourão, PR, 87301-006, Brazil., Ames FQ; Department of Pharmacology and Therapeutics, Bloco K68, State University of Maringá (UEM), Avenue Colombo, 5790, Jd. Universitário, 87020-900, Maringá, Brazil., Schneider LCL; Department of Morphological Sciences, State University of Maringá (UEM), Avenue Colombo, 5790, Jd. Universitário, Maringá, PR, 87020-900, Brazil., Duda JV; Department of Morphological Sciences, State University of Maringá (UEM), Avenue Colombo, 5790, Jd. Universitário, Maringá, PR, 87020-900, Brazil., Cardia GFE; Department of Pharmacology and Therapeutics, Bloco K68, State University of Maringá (UEM), Avenue Colombo, 5790, Jd. Universitário, 87020-900, Maringá, Brazil., Bonetti CI; Department of Biochemistry, State University of Maringá (UEM), Avenue Colombo, 5790, Jd. Universitário, Maringá, PR, 87020-900, Brazil., Cuman RKN; Department of Pharmacology and Therapeutics, Bloco K68, State University of Maringá (UEM), Avenue Colombo, 5790, Jd. Universitário, 87020-900, Maringá, Brazil. rkncuman@uem.br., Bersani-Amado CA; Department of Pharmacology and Therapeutics, Bloco K68, State University of Maringá (UEM), Avenue Colombo, 5790, Jd. Universitário, 87020-900, Maringá, Brazil.
Jazyk: angličtina
Zdroj: Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 2023 Mar; Vol. 396 (3), pp. 469-484. Date of Electronic Publication: 2022 Nov 17.
DOI: 10.1007/s00210-022-02323-2
Abstrakt: The present study prepared, optimized, and characterized solid lipid microparticles that contained trans-anethole (SLMAN), evaluated their antiinflammatory activity in acute and chronic inflammation models, and investigated their effects on the gastric mucosa in arthritic rats. The microparticles were obtained by a hot homogenization process and characterized by physicochemical analyses. The acute inflammatory response was induced by an intradermal injection of 0.1 ml of carrageenan solution (200 μg) in the hind paw. The rats were treated orally with a single dose of SLMAN 1 h before induction of the inflammatory response. The chronic inflammatory response was induced by the subcutaneous application of 0.1 ml of complete Freund's adjuvant suspension (500 µg) in the hind paw. SLMAN was orally administered, starting on the day of arthritis induction, and continued for 21 days. The results showed that SLMAN was obtained with good encapsulation efficiency. Treatment with SLMAN at doses of 25 and 50 mg/kg was as effective as trans-anethole (AN) at a dose of 250 mg/kg on acute and chronic inflammatory responses. Histological analyses showed that treatment with SLMAN did not aggravate lesions in the gastric mucosa in arthritic rats. These results indicated that treatment with SLMAN at a dose that was 5-10 times lower than non-encapsulated AN exerted an inhibitory effect on acute and chronic inflammatory responses, suggesting the better bioavailability and efficacy of microencapsulated AN without aggravating lesions in the gastric mucosa in arthritic rats.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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