Solanum lycopersicum CLASS-II KNOX genes regulate fruit anatomy via gibberellin-dependent and independent pathways.

Autor:	Shtern A; Institute of Plant Sciences, Agricultural Reserch Organization, Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel., Keren-Keiserman A; Institute of Plant Sciences, Agricultural Reserch Organization, Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel., Mauxion JP; UMR1332 Biologie du fruit et Pathologie, INRAE, University of Bordeaux, F33882 Villenave d'Ornon, France., Furumizu C; School of Biological Sciences, Monash University, Wellington Road, Clayton, Melbourne, VIC 3800, Australia., Alvarez JP; School of Biological Sciences, Monash University, Wellington Road, Clayton, Melbourne, VIC 3800, Australia.; ARC Centre of Excellence for Plant Success in Nature and Agriculture, Monash University, Wellington Road, Melbourne, VIC 3800, Australia., Amsellem Z; Department of Plant and Environmental Sciences, Weizmann Institute of Science, Herzel 234, Rehovot 7610001, Israel., Gil N; Department of Plant Science, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, PO Box 12, Rehovot 7610001, Israel., Motenko E; Institute of Plant Sciences, Agricultural Reserch Organization, Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel.; Department of Plant Science, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, PO Box 12, Rehovot 7610001, Israel., Alkalai-Tuvia S; Institute of Postharvest and Food Sciences, Agricultural Reserch Organization, Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel., Fallik E; Institute of Postharvest and Food Sciences, Agricultural Reserch Organization, Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel., Gonzalez N; UMR1332 Biologie du fruit et Pathologie, INRAE, University of Bordeaux, F33882 Villenave d'Ornon, France., Goldshmidt A; Institute of Plant Sciences, Agricultural Reserch Organization, Volcani Institute, HaMaccabbim Road 68, Rishon LeZion 7505101, Israel.
Jazyk:	angličtina
Zdroj:	Journal of experimental botany [J Exp Bot] 2023 Feb 05; Vol. 74 (3), pp. 848-863.
DOI:	10.1093/jxb/erac454
Abstrakt:	The pericarp is the predominant tissue determining the structural characteristics of most fruits. However, the molecular and genetic mechanisms controlling pericarp development remain only partially understood. Previous studies have identified that CLASS-II KNOX genes regulate fruit size, shape, and maturation in Arabidopsis thaliana and Solanum lycopersicum. Here we characterized the roles of the S. lycopersicum CLASS-II KNOX (TKN-II) genes in pericarp development via a detailed histological, anatomical, and karyotypical analysis of TKN-II gene clade mRNA-knockdown (35S:amiR-TKN-II) fruits. We identify that 35S:amiR-TKN-II pericarps contain more cells around their equatorial perimeter and fewer cell layers than the control. In addition, the cell sizes but not the ploidy levels of these pericarps were dramatically reduced. Further, we demonstrate that fruit shape and pericarp layer number phenotypes of the 35S:amiR-TKN-II fruits can be overridden by the procera mutant, known to induce a constitutive response to the plant hormone gibberellin. However, neither the procera mutation nor exogenous gibberellin application can fully rescue the reduced pericarp width and cell size phenotype of 35S:amiR-TKN-II pericarps. Our findings establish that TKN-II genes regulate tomato fruit anatomy, acting via gibberellin to control fruit shape but utilizing a gibberellin-independent pathway to control the size of pericarp cells. (© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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