Plasma Extracellular Vesicle miRNA Profiles Distinguish Chronic Obstructive Pulmonary Disease Exacerbations and Disease Severity.

Autor:	O'Farrell HE; Faculty of Medicine, the University of Queensland, Brisbane, QLD, Australia., Bowman RV; Faculty of Medicine, the University of Queensland, Brisbane, QLD, Australia., Fong KM; Faculty of Medicine, the University of Queensland, Brisbane, QLD, Australia., Yang IA; Faculty of Medicine, the University of Queensland, Brisbane, QLD, Australia.
Jazyk:	angličtina
Zdroj:	International journal of chronic obstructive pulmonary disease [Int J Chron Obstruct Pulmon Dis] 2022 Nov 04; Vol. 17, pp. 2821-2833. Date of Electronic Publication: 2022 Nov 04 (Print Publication: 2022).
DOI:	10.2147/COPD.S379774
Abstrakt:	Purpose: Molecular biomarkers for chronic obstructive pulmonary disease (COPD) severity have been difficult to identify. We aimed to assess extracellular vesicle miRNAs' potential as a blood biomarker in discriminating disease severity in participants with COPD. Patients and Methods: Plasma extracellular vesicles (EVs) were obtained from two COPD cohorts (n = 20 during an exacerbation event, n = 20 during stable state), with varying disease severity (GOLD stages). The miRCURY LNA miRNA Serum/Plasma assay, specific to 179 targets, was used to evaluate EV miRNA expression. The miRNAs that were significantly dysregulated were further assessed for discriminatory power using ROC curve analysis, as well as their role in relevant biological pathways. Results: One miRNA was significantly dysregulated between moderate GOLD participants compared to severe/very severe GOLD participants, with an AUC of 0.798, p = 0.01 for miR-374b-5p. Five miRNAs were significantly dysregulated between exacerbating and stable COPD participants, with miR-223-3p resulting in the highest AUC (0.755, p = 0.006) for a single miRNA, with a combination of three miRNAs (miR-92b-3p, miR-374a-5p and miR-106b-3p) providing the highest discriminatory power (AUC 0.820, p = 0.001). The "cytokine-cytokine receptor interaction" (hsa04060 pathway) was the most significant KEGG pathway enriched for three out of the five miRNAs associated with COPD exacerbations. Conclusion: This initial small-scale study suggests that the bioactive cargo (miRNAs) in plasma EVs holds specific biological information for the severity of airflow obstruction and COPD exacerbations, warranting further investigation. Competing Interests: Professor Kwun M Fong reports grants from NHMRC/MRFF, CCQ, ACRF, and TPCH Foundation; non-financial support from MeVis Veolity and Olympus, outside the submitted work. The authors report no other conflicts of interest in this work. (© 2022 O’Farrell et al.)
Databáze:	MEDLINE
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