Angiogenic and Inflammatory Alterations of Endometriotic Lesions in a Transgenic Animal Experimental Model With Loss of Expression of PPAR-Alpha Receptors.

Autor:	Pergialiotis V; First Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens, Athens, GRC., Zarkadoulas N; Laboratory of Experimental Surgery and Surgical Research N.S. Christeas, National and Kapodistrian University of Athens School of Medicine, Athens, GRC., Goula K; Department of Pathology, Alexandra Hospital, Athens, GRC., Frountzas M; First Propaedeutic Department of Surgery, Hippocration General Hospital, National and Kapodistrian University of Athens School of Medicine, Athens, GRC., Antoniadou F; Department of Pathology, Alexandra Hospital, Athens, GRC., Dimitroulis D; Second Department of Surgery, National and Kapodistrian University of Athens School of Medicine, Athens, GRC., Vlachos D; First Department of Obstetrics and Gynecology, National and Kapodistrian University of Athens School of Medicine, Athens, GRC., Papapanagiotou A; Department of Biological Chemistry, National and Kapodistrian University of Athens School of Medicine, Athens, GRC., Verikokos C; Second Department of Surgery, Laikon Hospital, National and Kapodistrian University of Athens School of Medicine, Athens, GRC., Perrea DN; Laboratory of Experimental Surgery and Surgical Research, National and Kapodistrian University of Athens School of Medicine, Athens, GRC., Kontzoglou K; Second Department of Surgery, Laikon Hospital, National and Kapodistrian University of Athens School of Medicine, Athens, GRC.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2022 Oct 14; Vol. 14 (10), pp. e30290. Date of Electronic Publication: 2022 Oct 14 (Print Publication: 2022).
DOI:	10.7759/cureus.30290
Abstrakt:	Introduction: Peroxisome proliferator-activated receptors (PPARs) have been proposed as a medical treatment against endometriosis in preclinical and clinical studies. Their effect seems to be triggered through the suppression of angiogenesis. In the present study, we used a transgenic animal model with a loss of expression of PPAR-alpha receptors to examine their effect on the course of surgically induced endometriotic lesions. Methods: Ten C57BL/6 mice that served as controls and 10 B6;129S4-PPARa tm1Gonz/J t transgenic mice characterized by absolute loss of expression of PPAR-alpha receptors were used for induction of endometriosis with a previously described surgical technique. Results: Five animals (50%) exhibited abundant endometriotic crypts in the control group whereas only one (10%) animal in the transgenic experimental group had a similar pathological image. Neo-vascularization significantly differed among the two groups (p=0.034) favoring the control group as it was extremely limited in half of the PPAR-alpha null animals. The median inflammation score was 2.5 (1-4) in the P B6;129S4-PPARa tm1Gonz/J group, whereas it was minimal, 1 (0-2), in the C57BL/6 group. However, these differences were not statistically significant (p=0.101). The fibroblastic activity was also very limited in the PPAR-alpha-deficient model, whereas animals belonging to the control group exhibited an intermediate increase of this index (p=0.022). Conclusion: Surgically induced endometriotic implants in animals with loss of expression of PPAR-alpha receptors exhibit significant differences in their pathology compared to lesions induced in control animals. This information suggests that PPAR-alpha receptors have a significant impact on the course of the disease, indicating that they may serve as potential targets for future medical therapies. Competing Interests: The authors have declared that no competing interests exist. (Copyright © 2022, Pergialiotis et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu