Crystal structure of semi-synthetic obelin-v after calcium induced bioluminescence implies coelenteramine as the main reaction product.

Autor: Natashin PV; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia., Eremeeva EV; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia.; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russia., Shevtsov MB; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Kovaleva MI; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Bukhdruker SS; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Dmitrieva DA; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Gulnov DV; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russia., Nemtseva EV; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia.; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russia., Gordeliy VI; Institut de Biologie Structurale (IBS), Université de Grenoble Alpes, CEA, CNRS, Grenoble, France.; Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany., Mishin AV; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Borshchevskiy VI; Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, Russia. borshchevskiy.vi@phystech.edu.; Joint Institute for Nuclear Research, Dubna, Russia. borshchevskiy.vi@phystech.edu., Vysotski ES; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia. eugene_vysotski@ibp.ru.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Nov 15; Vol. 12 (1), pp. 19613. Date of Electronic Publication: 2022 Nov 15.
DOI: 10.1038/s41598-022-24117-5
Abstrakt: Coelenterazine-v (CTZ-v), a synthetic vinylene-bridged π-extended derivative, is able to significantly alter bioluminescence spectra of different CTZ-dependent luciferases and photoproteins by shifting them towards longer wavelengths. However, Ca 2+ -regulated photoproteins activated with CTZ-v display very low bioluminescence activities that hampers its usage as a substrate of photoprotein bioluminescence. Here, we report the crystal structure of semi-synthetic Ca 2+ -discharged obelin-v bound with the reaction product determined at 2.1 Å resolution. Comparison of the crystal structure of Ca 2+ -discharged obelin-v with those of other obelins before and after bioluminescence reaction reveals no considerable changes in the overall structure. However, the drastic changes in CTZ-binding cavity are observed owing to the completely different reaction product, coelenteramine-v (CTM-v). Since CTM-v is certainly the main product of obelin-v bioluminescence and is considered to be a product of the "dark" pathway of dioxetanone intermediate decomposition, it explains the low bioluminescence activity of obelin and apparently of other photoproteins with CTZ-v.
(© 2022. The Author(s).)
Databáze: MEDLINE
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