Harnessing plant viruses in the metagenomics era: from the development of infectious clones to applications.

Autor: Shakir S; Plant Genetics and Rhizosphere Processes Laboratory, TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium. Electronic address: sshakir@uliege.be., Zaidi SS; Plant Genetics and Rhizosphere Processes Laboratory, TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium., Hashemi FSG; Plant Genetics and Rhizosphere Processes Laboratory, TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium., Nyirakanani C; Plant Genetics and Rhizosphere Processes Laboratory, TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium; Department of Crop Science, School of Agriculture, University of Rwanda, Musanze, Rwanda., Vanderschuren H; Plant Genetics and Rhizosphere Processes Laboratory, TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium; Laboratory of Tropical Crop Improvement, Division of Crop Biotechnics, Biosystems Department, KU Leuven, Leuven, Belgium. Electronic address: herve.vanderschuren@kuleuven.be.
Jazyk: angličtina
Zdroj: Trends in plant science [Trends Plant Sci] 2023 Mar; Vol. 28 (3), pp. 297-311. Date of Electronic Publication: 2022 Nov 12.
DOI: 10.1016/j.tplants.2022.10.005
Abstrakt: Recent metagenomic studies which focused on virus characterization in the entire plant environment have revealed a remarkable viral diversity in plants. The exponential discovery of viruses also requires the concomitant implementation of high-throughput methods to perform their functional characterization. Despite several limitations, the development of viral infectious clones remains a method of choice to understand virus biology, their role in the phytobiome, and plant resilience. Here, we review the latest approaches for efficient characterization of plant viruses and technical advances built on high-throughput sequencing and synthetic biology to streamline assembly of viral infectious clones. We then discuss the applications of plant viral vectors in fundamental and applied plant research as well as their technical and regulatory limitations, and we propose strategies for their safer field applications.
Competing Interests: Declaration of interests No interests are declared.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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