A continuous myofibroblast precursor cell line from the tail muscle of Australasian snapper (Chrysophrys auratus) that responds to transforming growth factor beta and fibroblast growth factor.

Autor: Chong GLW; The New Zealand Institute for Plant and Food Research Ltd, Nelson Research Centre, 293 Akersten Street, Nelson, 7010, New Zealand., Böhmert B; The New Zealand Institute for Plant and Food Research Ltd, Nelson Research Centre, 293 Akersten Street, Nelson, 7010, New Zealand., Lee LEJ; Faculty of Science, University of the Fraser Valley, Abbotsford, BC, V2S 7M8, Canada., Bols NC; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Dowd GC; The New Zealand Institute for Plant and Food Research Ltd, Nelson Research Centre, 293 Akersten Street, Nelson, 7010, New Zealand. Georgina.Dowd@plantandfood.co.nz.
Jazyk: angličtina
Zdroj: In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 2022 Dec; Vol. 58 (10), pp. 922-935. Date of Electronic Publication: 2022 Nov 15.
DOI: 10.1007/s11626-022-00734-2
Abstrakt: Chrysophrys auratus (Australasian snapper) is one of the largest and most valuable finfish from capture fisheries in New Zealand, yet no cell lines from this species are reported in the scientific literature. Here, we describe a muscle-derived cell line initiated from the tail of a juvenile snapper which has been designated CAtmus1PFR (Chrysophrys auratus, tail muscle, Plant & Food Research). The cell line has been passaged over 100 times in 3 years and is considered immortal. Cells are reliant on serum supplementation for proliferation and exhibit a broad thermal profile comparable to the eurythermic nature of C. auratus in vivo. The impact of exogenous growth factors, including insulin-like growth factors I and II (IGF-I and IGF-II), basic fibroblast growth factor (bFGF), and transforming growth factor beta (TGFβ), on cell morphology and proliferation was investigated. Insulin-like growth factors acted as mitogens and had minimal effect on cell morphology. TGFβ exposure resulted in CAtmus1PFR exhibiting a myofibroblast morphology becoming enlarged with actin bundling. This differentiation was confirmed through the expression of smooth muscle actin (sma), an increase in type 1 collagen (col1a) expression, and a loss of motility. Expression of col1a and sma was decreased when cells were exposed to bFGF, and no actin bundling was observed. These data indicate that CAtmus1PFR may be myofibroblastic precursor cells descending from mesenchymal progenitor cells present in the tail muscle myosepta.
(© 2022. The Author(s).)
Databáze: MEDLINE
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