Super-resolution re-scan second harmonic generation microscopy.

Autor: Stanciu SG; Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania., Hristu R; Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania., Stanciu GA; Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania., Tranca DE; Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania., Eftimie L; Pathology Department, Emergency Military Hospital, 010825 Bucharest, Romania., Dumitru A; Department of Pathology, Bucharest Emergency University Hospital, 050098 Bucharest, Romania.; Department of Pathology, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania., Costache M; Department of Pathology, Bucharest Emergency University Hospital, 050098 Bucharest, Romania.; Department of Pathology, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania., Stenmark HA; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, 0379 Oslo, Norway., Manders H; Confocal.nl BV, Science Park 406, 1098XG Amsterdam, The Netherlands., Cherian A; Confocal.nl BV, Science Park 406, 1098XG Amsterdam, The Netherlands., Tark-Dame M; Confocal.nl BV, Science Park 406, 1098XG Amsterdam, The Netherlands., Manders EMM; Confocal.nl BV, Science Park 406, 1098XG Amsterdam, The Netherlands.
Jazyk: angličtina
Zdroj: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Nov 22; Vol. 119 (47), pp. e2214662119. Date of Electronic Publication: 2022 Nov 14.
DOI: 10.1073/pnas.2214662119
Abstrakt: Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.
Databáze: MEDLINE
Externí odkaz: