Rapid Point-of-Care Electrochemical Sensor for the Detection of Cancer Tn Antigen Carbohydrate in Whole Unprocessed Blood.

Autor: M Silva S; ARC Centre of Excellence for Electromaterials Science, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn3122, Victoria, Australia.; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne3065, Victoria, Australia.; Iverson Health Innovation Research Institute, Swinburne University of Technology, Hawthorn3122, Victoria, Australia., Langley DP; Universal Biosensors, Rowville3178, Victoria, Australia., Cossins LR; Universal Biosensors, Rowville3178, Victoria, Australia., Samudra AN; Universal Biosensors, Rowville3178, Victoria, Australia., Quigley AF; School of Electrical and Biomedical Engineering, RMIT University, Melbourne3001, Victoria, Australia.; Department of Medicine, University of Melbourne, St. Vincent's Hospital, Melbourne3065, Victoria, Australia.; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne3065, Victoria, Australia., Kapsa RMI; School of Electrical and Biomedical Engineering, RMIT University, Melbourne3001, Victoria, Australia.; Department of Medicine, University of Melbourne, St. Vincent's Hospital, Melbourne3065, Victoria, Australia.; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne3065, Victoria, Australia., Tothill RW; Peter MacCallum Cancer Centre, Department of Clinical Pathology, University of Melbourne, Melbourne3010, Victoria, Australia., Greene GW; Institute for Frontier Materials and ARC Centre of Excellence for Electromaterials Science, Deakin University, Waurn Ponds3216, Victoria, Australia., Moulton SE; ARC Centre of Excellence for Electromaterials Science, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn3122, Victoria, Australia.; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne3065, Victoria, Australia.; Iverson Health Innovation Research Institute, Swinburne University of Technology, Hawthorn3122, Victoria, Australia.
Jazyk: angličtina
Zdroj: ACS sensors [ACS Sens] 2022 Nov 25; Vol. 7 (11), pp. 3379-3388. Date of Electronic Publication: 2022 Nov 14.
DOI: 10.1021/acssensors.2c01460
Abstrakt: Improving outcomes for cancer patients during treatment and monitoring for cancer recurrence requires personalized care which can only be achieved through regular surveillance for biomarkers. Unfortunately, routine detection for blood-based biomarkers is cost-prohibitive using currently specialized laboratories. Using a rapid self-assembly sensing interface amenable to methods of mass production, we demonstrate the ability to detect and quantify a small carbohydrate-based cancer biomarker, Tn antigen (αGalNAc-Ser/Thr) in a small volume of blood, using a test format strip reminiscent of a blood glucose test. The detection of Tn antigen at picomolar levels is achieved through a new transduction mechanism based on the impact of Tn antigen interactions on the molecular dynamic motion of a lectin cross-linked lubricin antifouling brush. In tests performed on retrospective blood plasma samples from patients presenting three different tumor types, differentiation between healthy and diseased patients was achieved, highlighting the clinical potential for cancer monitoring.
Databáze: MEDLINE
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