The prognostic role of C-KIT, TET1 and TET2 gene expression in Acute Myeloid Leukemia.

Autor: Nabil R; Clinical pathology Department, National Cancer Institute, Cairo University, Giza, Egypt., Hassan NM; Clinical pathology Department, National Cancer Institute, Cairo University, Giza, Egypt., Abdellateif MS; Medical Biochemistry and molecular biology, Cancer Biology Department, National Cancer Institute, Cairo University, Giza, Egypt. mona-sayed@cu.edu.eg., Gawdat RM; Clinical and chemical pathology department, Faculty of medicine, Beni Suef university, Beni Suef, Egypt., Elshazly SS; Clinical pathology Department, National Cancer Institute, Cairo University, Giza, Egypt.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2023 Jan; Vol. 50 (1), pp. 641-653. Date of Electronic Publication: 2022 Nov 12.
DOI: 10.1007/s11033-022-08000-0
Abstrakt: Aim: was to assess the role of C-KIT, TET1 and TET2 expression in the diagnosis and prognosis of acute myeloblastic leukemia (AML).
Methods: The expression levels of C-KIT, TET1 and TET2 were assessed in the bone marrow (BM) aspirate of 152 AML patients compared to 20 healthy control using quantitative real-time polymerase chain reaction (qRT-PCR). Data were correlated with the clinico-pathological features of the patients, response to treatment, disease-free survival (DFS), and overall survival (OS) rates.
Results: C-KIT, TET1 and TET2 were significantly upregulated in AML patients [0.25 (0-11.6), 0.0113 (0-3.301), and 0.07 (0-4); respectively], compared to the control group [0.013 (0.005-0.250), P < 0.001, 0.001 (0-0.006), P < 0.001, and 0.02 (0.008-0.055), P = 0.019; respectively]. The sensitivity, specificity, and area under curve of of C-KIT were (48.7%, 100%, 0.855; respectively, P = 0.001), and that of TET1 were (63.4%, 100%, 0.897; respectively, P = 0.001), while that of TET2 were (56.8%, 100%, 0.766; respectively, P = 0.019). When combining the three markers, the sensitivity was 77.5%, however it reached the highest sensitivity (78.6%) and specificity (100%) when combining both c-KIT + TET1 together for the diagnosis of AML. C-KIT overexpression associated with shorter DFS (P = 0.05) and increased incidence of relapse (P = 0.019). Lymph nodes involvement [HR = 2.200, P = 0.005] is an independent risk factor for shorter OS rate of AML patients. Increased BM blast % [HR = 7.768, P = 0.002], and FLT3-ITD mutation [HR = 2.989, P = 0.032] are independent risk factors for shorter DSF rate of the patients.
Conclusion: C-KIT, TET1, and TET2 could be used as possible useful biomarkers for the diagnosis of AML.
(© 2022. The Author(s).)
Databáze: MEDLINE
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