Clinical evaluation of a Borrelia modified two-tiered testing (MTTT) shows increased early sensitivity for Borrelia burgdorferi but not other endemic Borrelia species in a high incidence region for Lyme disease in Wisconsin.

Autor: Sabin AP; Division of Infectious Diseases, Gundersen Health System, La Crosse, WI, USA; Microbiology Research Laboratory, Gundersen Medical Foundation, La Crosse, WI, USA. Electronic address: apsabin@gundersenhealth.org., Scholze BP; Microbiology Research Laboratory, Gundersen Medical Foundation, La Crosse, WI, USA., Lovrich SD; Microbiology Research Laboratory, Gundersen Medical Foundation, La Crosse, WI, USA., Callister SM; Microbiology Research Laboratory, Gundersen Medical Foundation, La Crosse, WI, USA.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2023 Jan; Vol. 105 (1), pp. 115837. Date of Electronic Publication: 2022 Oct 17.
DOI: 10.1016/j.diagmicrobio.2022.115837
Abstrakt: A modified two-tiered testing algorithm (MTTT; ZEUS Scientific) for Borrelia burgdorferi was recently FDA-cleared. We evaluated the MTTT algorithm to confirm Lyme disease and compared the findings in parallel with those obtained using standard two-tiered testing (STTT). Medical records from patients who submitted sera for laboratory confirmation of Lyme-like disease were reviewed. Three hundred twenty patient samples were run by the STTT and MTTT approaches and the results compared. Positive STTT samples were also positive by MTTT (94%). The MTTT confirmed the illness in 116 subjects (36%, P = 0.007), and 30 (26%) were negative by the STTT. Increased MTTT sensitivity was seen (P = 0.0005) during early infection. MTTT was insufficiently sensitive to identify other non-Borrelia spp. infections. Routine adoption of MTTT would improve sensitivity for early Lyme disease attributable to B. burgdorferi, but may not capture illness attributed to B. mayonii and B. miyamotoi.
Competing Interests: Declaration of competing interest The authors (Arick P. Sabin [corresponding], Brooklynn P. Scholze, Steven D. Lovrich, and Steven M. Callister) have no financial or other conflicts of interest to disclose as it pertains to this manuscript.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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