SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe.
| Autor: | Anrather D; Mass Spectrometry Facility, Max Perutz Labs, Vienna BioCenter (VBC), Vienna, Austria. dorothea.anrather@univie.ac.at., Polakova SB; Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Bratislava, Slovakia. silvia.bagelova@savba.sk.; Department of Genetics, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovakia. silvia.bagelova@savba.sk., Cipak L; Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia. lubos.cipak@savba.sk., Gregan J; Advanced Microscopy Facility, VBCF and Department of Chromosome Biology, Max Perutz Labs, University of Vienna, Vienna BioCenter (VBC), Vienna, Austria. juraj.gregan@univie.ac.at. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2603, pp. 19-29. |
| DOI: | 10.1007/978-1-0716-2863-8_2 |
| Abstrakt: | Stable isotope labeling by amino acids in cell culture (SILAC) provides a powerful tool to quantify proteins and posttranslational modifications. Here we describe how to apply SILAC for protein identification and quantification in synchronous meiotic cultures induced by inactivation of the Pat1 kinase in the fission yeast Schizosaccharomyces pombe. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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