Inhibition of Klebsiella pneumoniae carbapenemases by mycocins produced by Wickerhamomyces anomalus.

Autor: Nascimento BL; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil., Martelli EC; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil., da Silva JC; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil., Delabeneta MF; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil., Rosseto LRB; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil., Junges DSB; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil., Paris AP; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil., Persel C; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil., Paula CR; Faculty of Dentistry, Department of Stomatology, University of São Paulo, São Paulo, São Paulo, Brazil., Simão RCG; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil., Gandra RF; University Hospital From Western Paraná State University, At 3224, Tancredo Neves Avenue, Cascavel City, Paraná, 85806-470, Brazil. rinaldo.gandra@unioeste.br.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2022 Nov 12; Vol. 204 (12), pp. 702. Date of Electronic Publication: 2022 Nov 12.
DOI: 10.1007/s00203-022-03311-z
Abstrakt: New alternatives have been under study as treatment due to the problem of multidrug-resistant bacteria. Among them, Wickerhamomyces anomalus mycocins have shown a great potential against several microorganisms since they have high antimicrobial activity, as well as they can be used as fast available nutrients and stand several extreme conditions. In this way, Klebsiella pneumoniae carbapenemases inhibition by mycocins produced by W. anomalus is important. Microdilution assays were carried out to evaluate strains inhibition in liquid medium and the test in solid medium were carried out. Toxicity was evaluated by both hemolysis and Artemia salina Leach tests. W. anomalus supernatant showed 2.36 U/mg β-glucanases activity, and antimicrobial activity was evidenced in 100% Klebsiella pneumoniae carbapenemase strains up to 0.12 U/mg concentration. Besides, there was low toxicity in hemolysis and Artemia salina Leach tests. It is suggested that W. anomalus mycocins may be an alternative to develop new antimicrobial substances.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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