Laminin111-based defined culture promoting self-renewing human pluripotent stem cells with properties of the early post-implantation epiblast.

Autor:	Gropp M; The Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel., Waldhorn I; The Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel., Gil Y; The Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel., Steiner D; The Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel., Turetsky TT; The Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel., Smith Y; The Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel., Sabag O; Department of Developmental Biology and Cancer Research, Faculty of Medicine, Hebrew University of Jerusalem, Israel., Falick-Michaeli T; Rubin Chair in Medical Science, Department of Developmental Biology & Cancer Research, IMRIC, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel., Even Ram S; The Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel., Reubinoff BE; The Hadassah Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel; Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 91120 Jerusalem, Israel. Electronic address: benr@hadassah.org.il.
Jazyk:	angličtina
Zdroj:	Stem cell reports [Stem Cell Reports] 2022 Dec 13; Vol. 17 (12), pp. 2643-2660. Date of Electronic Publication: 2022 Nov 10.
DOI:	10.1016/j.stemcr.2022.10.010
Abstrakt:	In the mammalian embryo, a formative pluripotent phase is proposed to exist at the early post-implantation period, during the transition from the pre-implantation naive-to the post-implantation primed-epiblast. By recapitulating a laminin component of the extracellular matrix niche during embryonic formative transition, and defined culture conditions, we generated cultures highly enriched for self-renewing human pluripotent stem cells (hPSCs), exhibiting properties of early post-implantation epiblast cells. These hPSCs display post-implantation-epiblast gene expression profiles. FGF and TGF-β signaling maintain their self-renewal for multiple passages. They have inactive canonical Wnt signaling, do not express primitive streak markers, and are competent to initiate differentiation toward germline and somatic fates. hPSCs exhibiting early post-implantation epiblast properties may shed light on human embryonic PSCs development and may serve for initiating somatic and germ cell specification. Competing Interests: Conflict of interests B.E.R. is a member of the journal’s Editorial Board. He is a founder, holds shares, and is the Chief Scientific Officer of CellCure Neuroscience Ltd. The company did not fund the study presented in this manuscript and has no interest in its results. A patent application related to the data presented in this manuscript has been submitted. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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