Retrospective Analysis of the SARS-CoV-2 Infection Profile in COVID-19 Positive Patients in Vitoria da Conquista, Northeast Brazil.

Autor:	Dantas ACS; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil.; Municipal Central Laboratory, Vitória da Conquista City Hall, Avenida Macaúbas, 100, Kadija, Vitória da Conquista 45065-060, Bahia, Brazil., Oliveira HBM; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil., Gomes CP; State University of Santa Cruz, Rod. Jorge Amado, Km 6, Salobrinho, Ilhéus 55662-900, Bahia, Brazil., Alves DL; Municipal Central Laboratory, Vitória da Conquista City Hall, Avenida Macaúbas, 100, Kadija, Vitória da Conquista 45065-060, Bahia, Brazil., Infante PDB; Municipal Central Laboratory, Vitória da Conquista City Hall, Avenida Macaúbas, 100, Kadija, Vitória da Conquista 45065-060, Bahia, Brazil., Caitité RJA; Municipal Central Laboratory, Vitória da Conquista City Hall, Avenida Macaúbas, 100, Kadija, Vitória da Conquista 45065-060, Bahia, Brazil., Fritsch HM; Oswaldo Cruz Institute-Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro 21040-900, Rio de Janeiro, Brazil., Cucco MS; Medical School, Federal University of Bahia, Largo Terreiro de Jesus, s/n, Pelourinho, Salvador 40026-010, Bahia, Brazil., Silva LSC; State University of Santa Cruz, Rod. Jorge Amado, Km 6, Salobrinho, Ilhéus 55662-900, Bahia, Brazil., Oliveira CNT; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil., Bittencourt RS; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil., Amorim AT; Northeast Independent College, Avenida Luís Eduardo Magalhães, 1305, Candeias, Vitória da Conquista 45055-030, Bahia, Brazil., Nascimento ALP; Northeast Independent College, Avenida Luís Eduardo Magalhães, 1305, Candeias, Vitória da Conquista 45055-030, Bahia, Brazil., Marinho FAGC; Northeast Independent College, Avenida Luís Eduardo Magalhães, 1305, Candeias, Vitória da Conquista 45055-030, Bahia, Brazil., de Medeiros DS; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil., de Oliveira MGG; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil., Mistro S; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil., de Melo FF; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil., Pereira TTS; Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã, São Paulo 05508-900, Brazil., Guimarães AMS; Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã, São Paulo 05508-900, Brazil., Timenetsky J; Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 2415, Butantã, São Paulo 05508-900, Brazil., Moreira PMB; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil.; Vitória da Conquista City Hall, Rua Rotary Club, 69-Centro, Vitória da Conquista 45040-150, Bahia, Brazil., de Oliveira SHP; Júlio de Mesquita Filho State University of São Paulo, Campus Universitário, Rodovia Marechal Rondon km 527/528, Araçatuba 16015050, Brazil., Alcantara LCJ; Oswaldo Cruz Institute-Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro 21040-900, Rio de Janeiro, Brazil., Giovanetti M; Oswaldo Cruz Institute-Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro 21040-900, Rio de Janeiro, Brazil.; University of Campus Bio-Medico di Roma, Rome, 00128, Italy., Santos LA; Medical School, Federal University of Bahia, Largo Terreiro de Jesus, s/n, Pelourinho, Salvador 40026-010, Bahia, Brazil.; Bahia School of Medicine and Public Heath, Av. Dom João VI, 275, Brotas, Salvador 40290-000, Bahia, Brazil., Fonseca V; Pan American Health Organization. Lote 19-Avenida das Nações, SEN-Asa Norte, Brasilia 70312-970, Brazil., Barreto FK; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil., Campos GB; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil., Marques LM; Institute of Multidisciplinary Health, Federal University of Bahia, Rua Hormindo Barros, 58, Candeias, Vitória da Conquista 45029-094, Bahia, Brazil.; Municipal Central Laboratory, Vitória da Conquista City Hall, Avenida Macaúbas, 100, Kadija, Vitória da Conquista 45065-060, Bahia, Brazil.
Jazyk:	angličtina
Zdroj:	Viruses [Viruses] 2022 Oct 31; Vol. 14 (11). Date of Electronic Publication: 2022 Oct 31.
DOI:	10.3390/v14112424
Abstrakt:	Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is responsible for causing Coronavirus Disease-2019 (COVID-19), a heterogeneous clinical condition that manifests varying symptom severity according to the demographic profile of the studied population. While many studies have focused on the spread of COVID-19 in large urban centers in Brazil, few have evaluated medium or small cities in the Northeast region. The aims of this study were: (i) to identify risk factors for mortality from SARS-CoV-2 infection, (ii) to evaluate the gene expression patterns of key immune response pathways using nasopharyngeal swabs of COVID-19 patients, and (iii) to identify the circulating SARS-CoV-2 variants in the residents of a medium-sized city in Northeast Brazil. A total of 783 patients infected with SARS-CoV-2 between May 2020 and August 2021 were included in this study. Clinical-epidemiological data from patients who died and those who survived were compared. Patients were also retrospectively divided into three groups based on disease severity: asymptomatic, mild, and moderate/severe. Samples were added to a qPCR array for analyses of 84 genes involved with immune response pathways and sequenced using the Oxford Nanopore MinION technology. Having pre-existing comorbidity; being male; having cardiovascular disease, diabetes, and/or chronic obstructive pulmonary disease; and PCR cycle threshold (Ct) values under 22 were identified as risk factors for mortality. Analysis of the expression profiles of inflammatory pathway genes showed that the greater the infection severity, the greater the activation of inflammatory pathways, triggering the cytokine storm and downregulating anti-inflammatory pathways. Viral genome analysis revealed the circulation of multiple lineages, such as B.1, B.1.1.28, Alpha, and Gamma, suggesting that multiple introduction events had occurred over time. This study's findings help identify the specific strains and increase our understanding of the true state of local health. In addition, our data demonstrate that epidemiological and genomic surveillance together can help formulate public health strategies to guide governmental actions.
Databáze:	MEDLINE
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