| Autor: |
Etxeberria L; Leartiker S. Coop., Xemein Etorbidea 12, 48270 Markina-Xemein, Spain.; microLIQUID S.L, Goiru 9, 20500 Arrasate-Mondragon, Spain.; Macromolecular Chemistry Research Group (labquimac), Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain., Messelmani T; CNRS, Biomechanics and Bioengineering, Centre de Recherche Royallieu-CS 60319, Université de Technologie de Compiègne, 60203 Compiègne, France., Badiola JH; Leartiker S. Coop., Xemein Etorbidea 12, 48270 Markina-Xemein, Spain., Llobera A; microLIQUID S.L, Goiru 9, 20500 Arrasate-Mondragon, Spain., Fernandez L; microLIQUID S.L, Goiru 9, 20500 Arrasate-Mondragon, Spain., Vilas-Vilela JL; Macromolecular Chemistry Research Group (labquimac), Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Spain.; BC Materials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain., Leclerc E; CNRS, Biomechanics and Bioengineering, Centre de Recherche Royallieu-CS 60319, Université de Technologie de Compiègne, 60203 Compiègne, France.; CNRS IRL 2820, Laboratory for Integrated Micro Mechatronic Systems, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Tokyo 153-8505, Japan., Legallais C; CNRS, Biomechanics and Bioengineering, Centre de Recherche Royallieu-CS 60319, Université de Technologie de Compiègne, 60203 Compiègne, France., Jellali R; CNRS, Biomechanics and Bioengineering, Centre de Recherche Royallieu-CS 60319, Université de Technologie de Compiègne, 60203 Compiègne, France., Zaldua AM; Leartiker S. Coop., Xemein Etorbidea 12, 48270 Markina-Xemein, Spain. |
| Abstrakt: |
Organ-on-chip (OoC) technology is one of the most promising in vitro tools to replace the traditional animal experiment-based paradigms of risk assessment. However, the use of OoC in drug discovery and toxicity studies remain still limited by the low capacity for high-throughput production and the incompatibility with standard laboratory equipment. Moreover, polydimethylsiloxanes, the material of choice for OoC, has several drawbacks, particularly the high absorption of drugs and chemicals. In this work, we report the development of a microfluidic device, using a process adapted for mass production, to culture liver cell line in dynamic conditions. The device, made of cyclic olefin copolymers, was manufactured by injection moulding and integrates Luer lock connectors compatible with standard medical and laboratory instruments. Then, the COC device was used for culturing HepG2/C3a cells. The functionality and behaviour of cultures were assessed by albumin secretion, cell proliferation, viability and actin cytoskeleton development. The cells in COC device proliferated well and remained functional for 9 days of culture. Furthermore, HepG2/C3a cells in the COC biochips showed similar behaviour to cells in PDMS biochips. The present study provides a proof-of-concept for the use of COC biochip in liver cells culture and illustrate their potential to develop OoC. |
