| Autor: |
Algammal AM; Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt., Ibrahim RA; National Institute of Oceanography and Fisheries, Cairo 11516, Egypt., Alfifi KJ; Department of Biology, Faculty of Science, University of Tabuk, Tabuk 71421, Saudi Arabia., Ghabban H; Department of Biology, Faculty of Science, University of Tabuk, Tabuk 71421, Saudi Arabia., Alghamdi S; Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 21955, Saudi Arabia., Kabrah A; Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 21955, Saudi Arabia., Khafagy AR; Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt., Abou-Elela GM; National Institute of Oceanography and Fisheries, Cairo 11516, Egypt., Abu-Elala NM; Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.; Faculty of Veterinary Medicine, King Salman International University, El Tor 46612, Egypt., Donadu MG; Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy.; Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy., El-Tarabili RM; Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt. |
| Jazyk: |
angličtina |
| Zdroj: |
Pathogens (Basel, Switzerland) [Pathogens] 2022 Oct 29; Vol. 11 (11). Date of Electronic Publication: 2022 Oct 29. |
| DOI: |
10.3390/pathogens11111262 |
| Abstrakt: |
Aeromonas veronii is associated with substantial economic losses in the fish industry and with food-borne illness in humans. This study aimed to determine the prevalence, antibiogram profiles, sequence analysis, virulence and antimicrobial resistance genes, and pathogenicity of A. veronii recovered from Mugil seheli . A total of 80 fish were randomly gathered from various private farms in Suez Province, Egypt. Subsequently, samples were subjected to clinical, post-mortem, and bacteriological examinations. The retrieved isolates were tested for sequence analysis, antibiogram profile, pathogenicity, and PCR detection of virulence and resistance genes. The prevalence of A. veronii in the examined M. seheli was 22.5 % (18/80). The phylogenetic analyses revealed that the tested A. veronii strains shared high genetic similarity with other A. veronii strains from India, UK, and China. Using PCR it was revealed that the retrieved A. veronii isolates harbored the aer A, alt , ser , omp AII, act , ahp , and nuc virulence genes with prevalence of 100%, 82.9%, 61.7%, 55.3%, 44.7%, 36.17%, and 29.8%, respectively. Our findings revealed that 29.8% (14/47) of the retrieved A. veronii strains were XDR to nine antimicrobial classes and carried bla TEM , bla CTX-M , bla SHV, tet A, aad A1, and sul 1 resistance genes. Likewise, 19.1% (9/47) of the obtained A. veronii strains were MDR to eight classes and possessed bla TEM , bla CTX-M , bla SHV, tet A, aad A1, and sul 1 genes. The pathogenicity testing indicated that the mortality rates positively correlated with the prevalence of virulence-determinant genes. To our knowledge, this is the first report to reveal the occurrence of XDR and MDR A. veronii in M. seheli , an emergence that represents a risk to public health. Emerging XDR and MDR A. veronii in M. seheli frequently harbored aer A, alt , ser , omp AII, and act virulence genes, and bla TEM , sul 1, tet A, bla CTX-M , bla SHV , and aad A1 resistance genes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
