Lithographic SERS Aptasensor for Ultrasensitive Detection of SARS-CoV-2 in Biological Fluids.

Autor: Kukushkin V; Osipyan Institute of Solid State Physics of Russian Academy of Science, 142432 Chernogolovka, Russia., Ambartsumyan O; Department of Microbiology, Virology and Immunology, I.M. Sechenov First Moscow State Medical University, 125009 Moscow, Russia., Astrakhantseva A; Osipyan Institute of Solid State Physics of Russian Academy of Science, 142432 Chernogolovka, Russia.; Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia., Gushchin V; N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology, 123098 Moscow, Russia., Nikonova A; Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia., Dorofeeva A; Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia., Zverev V; Department of Microbiology, Virology and Immunology, I.M. Sechenov First Moscow State Medical University, 125009 Moscow, Russia.; Mechnikov Research Institute of Vaccines and Sera, 105064 Moscow, Russia., Gambaryan A; Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products RAS, 108819 Moscow, Russia., Tikhonova D; Osipyan Institute of Solid State Physics of Russian Academy of Science, 142432 Chernogolovka, Russia.; Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia., Sovetnikov T; Faculty of Physics, Lomonosov Moscow State University, 119991 Moscow, Russia., Akhmetova A; Faculty of Physics, Lomonosov Moscow State University, 119991 Moscow, Russia., Yaminsky I; Faculty of Physics, Lomonosov Moscow State University, 119991 Moscow, Russia., Zavyalova E; Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia.
Jazyk: angličtina
Zdroj: Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2022 Nov 01; Vol. 12 (21). Date of Electronic Publication: 2022 Nov 01.
DOI: 10.3390/nano12213854
Abstrakt: In this paper, we propose a technology for the rapid and sensitive detection of the whole viral particles of SARS-CoV-2 using double-labeled DNA aptamers as recognition elements together with the SERS method for detecting the optical response. We report on the development of a SERS-aptasensor based on a reproducible lithographic SERS substrate, featuring the combination of high speed, specificity, and ultrasensitive quantitative detection of SARS-CoV-2 virions. The sensor makes it possible to identify SARS-CoV-2 in very low concentrations (the limit of detection was 100 copies/mL), demonstrating a sensitivity level comparable to the existing diagnostic golden standard-the reverse transcription polymerase chain reaction.
Databáze: MEDLINE