| Autor: |
Zhgun A; Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia., Avdanina D; Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia., Shagdarova B; Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia., Nuraeva G; Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia., Shumikhin K; State Tretyakov Gallery, 119017 Moscow, Russia., Zhuikova Y; Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia., Il'ina A; Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia., Troyan E; State Tretyakov Gallery, 119017 Moscow, Russia., Shitov M; State Tretyakov Gallery, 119017 Moscow, Russia., Varlamov V; Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia. |
| Abstrakt: |
Microorganisms are one of the main factors in the deterioration of cultural heritage, in particular art paintings. The antiseptics currently used in painting have significant limitations due to insufficient effectiveness or increased toxicity and interaction with art materials. In this regard, the actual challenge is the search for novel materials that effectively work against microorganisms in the composition with painting materials and do not change their properties. Chitosan has pronounced antimicrobial properties but was not used previously as an antiseptic for paintings. In our study we developed a number of mock layers based on sturgeon glue, supplemented which chitosan (molecular weight 25 kDa or 45 kDa), standard antiseptics for paintings (positive controls) or without additives (negative control). According to Fourier transform infrared spectroscopy and atomic force microscopy, the addition of chitosan did not significantly affect the optical and surface properties of this material. The ability of chitosan to effectively protect paintings was shown after inoculation on the created mock-up layers of 10 fungi-destructors of tempera painting, previously isolated from cultural heritage of the of the 15-16th centuries in the State Tretyakov Gallery, on the created mock layers. Our study demonstrated the principled opportunity of using chitosan in the composition of painting materials to prevent biodeterioration for the first time. |
