| Autor: |
Simonazzi M; Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Via S'Alberto 163, 48123 Ravenna, Italy., Pezzolesi L; Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Via S'Alberto 163, 48123 Ravenna, Italy.; Interdepartmental Centre for Industrial Research in Renewable Resources, Environment, Sea and Energy (CIRI-FRAME), University of Bologna, Via S'Alberto 163, 48123 Ravenna, Italy., Guerrini F; Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Via S'Alberto 163, 48123 Ravenna, Italy., Vanucci S; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale Ferdinando d'Alcontres 31, 98166 Messina, Italy., Graziani G; Romagna Acque Società delle Fonti S.p.a., Piazza Orsi Mangelli 10, 47122 Forlì, Italy., Vasumini I; Romagna Acque Società delle Fonti S.p.a., Piazza Orsi Mangelli 10, 47122 Forlì, Italy., Pandolfi A; Romagna Acque Società delle Fonti S.p.a., Piazza Orsi Mangelli 10, 47122 Forlì, Italy., Servadei I; Fondazione Centro Ricerche Marine, Viale A. Vespucci, 2, 47042 Cesenatico, Italy., Pistocchi R; Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Via S'Alberto 163, 48123 Ravenna, Italy.; Interdepartmental Centre for Industrial Research in Renewable Resources, Environment, Sea and Energy (CIRI-FRAME), University of Bologna, Via S'Alberto 163, 48123 Ravenna, Italy. |
| Abstrakt: |
The use of multi-wavelength spectrofluorometers for the fast detection of algal taxa, based on chlorophyll a (Chl- a ) emission spectra, has become a common practice in freshwater water management, although concerns about their accuracy have been raised. Here, inter-laboratory comparisons using monoalgal cultures have been performed to assess the reliability of different spectrofluorometer models, alongside Chl- a extraction methods. Higher Chl- a concentrations were obtained when using the spectrofluorometers than extraction methods, likely due to the poor extraction efficiencies of solvents, highlighting that traditional extraction methods could underestimate algal or cyanobacterial biomass. Spectrofluorometers correctly assigned species to the respective taxonomic group, with low and constant percent attribution errors (Chlorophyta and Euglenophyceae 6-8%, Cyanobacteria 0-3%, and Bacillariophyta 10-16%), suggesting that functioning limitations can be overcome by spectrofluorometer re-calibration with fresh cultures. The monitoring of a natural phytoplankton assemblage dominated by Chlorophyta and Cyanobacteria gave consistent results among spectrofluorometers and with microscopic observations, especially when cell biovolume rather than cell density was considered. In conclusion, multi-wavelength spectrofluorometers were confirmed as valid tools for freshwater monitoring, whereas a major focus on intercalibration procedures is encouraged to improve their reliability and broaden their use as fast monitoring tools to prevent environmental and public health issues related to the presence of harmful cyanobacteria. |
