| Autor: |
Omori K; Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA., Gonzalez I; Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA., Nguyen C; Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA., Raminani SN; Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA., Deleon VM; Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA., Meza P; Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA., Zamalloa J; Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA., Perez RG; Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA., Gonzalez N; Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA., Komatsu H; Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA., Al-Abdullah IH; Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA., Wen X; Department of Chemistry and Biochemistry, California State University Los Angeles, Los Angeles, CA 90032, USA. |
| Abstrakt: |
Hypothermic (cold) preservation is a limiting factor for successful cell and tissue transplantation where cell swelling (edema) usually develops, impairing cell function. University of Wisconsin (UW) solution, a standard cold preservation solution, contains effective components to suppress hypothermia-induced cell swelling. Antifreeze proteins (AFPs) found in many cold-adapted organisms can prevent cold injury of the organisms. Here, the effects of a beetle AFP from Dendroides canadensis (DAFP-1) on pancreatic β-cells preservation were first investigated. As low as 500 µg/mL, DAFP-1 significantly minimized INS-1 cell swelling and subsequent cell death during 4 °C preservation in UW solution for up to three days. However, such significant cytoprotection was not observed by an AFP from Tenebrio molitor (TmAFP), a structural homologue to DAFP-1 but lacking arginine, at the same levels. The cytoprotective effect of DAFP-1 was further validated with the primary β-cells in the isolated rat pancreatic islets in UW solution. The submilligram level supplement of DAFP-1 to UW solution significantly increased the islet mass recovery after three days of cold preservation followed by rewarming. The protective effects of DAFP-1 in UW solution were discussed at a molecular level. The results indicate the potential of DAFP-1 to enhance cell survival during extended cold preservation. |
