| Autor: |
El Sherif HM; Department of Microbiology, Faculty of Pharmacy, Misr International University (MIU), Cairo 19648, Egypt., Elsayed M; Department of Clinical Pharmacy, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Cairo 12055, Egypt., El-Ansary MR; Department of Biochemistry, Modern University for Technology and Information (MTI), Cairo 12055, Egypt., Aboshanab KM; Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street, Cairo 11566, Egypt., El Borhamy MI; Department of Microbiology, Faculty of Pharmacy, Misr International University (MIU), Cairo 19648, Egypt.; International Medical Center, Clinical Microbiology Laboratory, Cairo 19648, Egypt., Elsayed KM; Department of Microbiology, Faculty of Pharmacy, Misr International University (MIU), Cairo 19648, Egypt. |
| Abstrakt: |
Central line-associated bloodstream infection (CLABSI) is among the most serious hospital acquired infections. Therefore, the rapid detection of the causative microorganism is of crucial importance to allow for the appropriate antimicrobial therapy. In the present study, we analyzed the clinical performance of the BioFire FilmArray Blood Culture Identification 2 (BCID2) panel in the identification of 33 microbial species and 10 antibiotic resistance genes in comparison to the VITEK-2 system. A total of 104 blood specimens were included. The FilmArray BCID2 results were concordant with the VITEK-2 system in 69/97 specimens (71.1%). Non-concordance was either due to the detection of more pathogens by the FilmArray BCID2 23/28 (82%) or microbial species were misidentified 5/28 (18%). Hence, in comparison to the VITEK-2 system, the FilmArray BCID2 panel showed an overall sensitivity of 75.8% (95% CI, 66-83%) and an overall specificity of 98% (95% CI, 97-98.8%) in detecting microbial species. For the resistance genes, the FilmArray BCID was able to detect the presence of bla CTX-M gene in 23 Gram-negative isolates, bla NDM and bla OXA-48- like genes in 14 and 13 isolates, respectively. The mec A and mec C genes were found in 23 Staphylococcus species, while mec A, mec C and MREJ genes were found in 4 Staphylococcus aureus isolates. The sensitivity and specificity for detecting resistance genes by the FilmArray BCID2 was 90% (95% CI, 81.4-95%) and 99.6% (95% CI, 99-100%), respectively. As concluded, the present study emphasizes the high sensitivity and specificity of the FilmArray BCID2 in the rapid and reliable detection of different bacteria and fungi from positive blood culture bottles, as well as the accurate detection of various antibiotic resistance markers. |
