Isolation of Chondrons from Hyaline Cartilage.
| Autor: | Korpershoek JV; Department of Orthopaedics, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands., Rikkers M; Department of Orthopaedics, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands., Vonk LA; Department of Orthopaedics, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands. lavonk@gmail.com.; CO.DON AG, Teltow, Germany. lavonk@gmail.com.; Xintela AB, Lund, Sweden. lavonk@gmail.com. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2598, pp. 21-27. |
| DOI: | 10.1007/978-1-0716-2839-3_3 |
| Abstrakt: | In native healthy hyaline cartilage, the chondrocytes are surrounded by a pericellular matrix that has a distinct composition and function compared to the hyaline cartilage extracellular matrix. The chondrocyte together with its pericellular matrix is called a chondron. The type VI collagen, which is the main component of the pericellular matrix, is resistant to enzymatic digestion by pure collagenase and dispase that do digest the extracellular matrix. Therefore, this combination of enzymes can be used to enzymatically isolate chondrons from hyaline cartilage. Chondrons have a high potential for cartilage tissue engineering. This chapter describes in detail how chondrons can be isolated from hyaline cartilage for further use. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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