| Autor: |
Foratori-Junior GA; Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil.; Centre for Host-Microbiome Interactions, Faculty of Dental, Oral & Craniofacial Sciences, Guy's Campus, King's College London, London SE1 1UL, UK., Guennec AL; Nuclear Magnetic Resonance Facility, Guy's Campus, King's College London, London SE1 1UL, UK., Fidalgo TKDS; Department of Preventive and Community Dentistry, School of Dentistry, Rio de Janeiro State University, Rio de Janeiro 20551-030, Brazil., Cleaver L; Centre for Host-Microbiome Interactions, Faculty of Dental, Oral & Craniofacial Sciences, Guy's Campus, King's College London, London SE1 1UL, UK., Buzalaf MAR; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil., Carpenter GH; Centre for Host-Microbiome Interactions, Faculty of Dental, Oral & Craniofacial Sciences, Guy's Campus, King's College London, London SE1 1UL, UK., Sales-Peres SHC; Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, Brazil. |
| Abstrakt: |
This study aimed to elucidate the metabolomic signature associated with obesity and periodontitis during pregnancy in plasma and saliva biofluids. Ninety-eight pregnant women were divided into: with obesity and periodontitis (OP = 20), with obesity but without periodontitis (OWP = 27), with normal BMI but with periodontitis (NP = 21), with normal BMI and without periodontitis (NWP = 30). Saliva and plasma were analyzed by 1H-NMR for metabolites identification. Partial Least Squares-Discriminant Analysis (PLS-DA), Sparse PLS-DA (sPLS-DA), and Variable Importance of Projection (VIP) were performed. ANOVA and Pearson’s correlation were applied (p < 0.05). Plasmatic analysis indicated the levels of glucose (p = 0.041) and phenylalanine (p = 0.015) were positively correlated with periodontal parameters and BMI, respectively. In saliva, periodontitis was mainly associated with high levels of acetic acid (p = 0.024), isovaleric acid, butyric acid, leucine, valine, isoleucine, and propionic acid (p < 0.001). High salivary concentrations of glycine (p = 0.015), succinic acid (p = 0.015), and lactate (p = 0.026) were associated with obesity. Saliva demonstrated a more elucidative difference than plasma, indicating the glucose-alanine cycle, alanine metabolism, valine, leucine and isoleucine degradation, glutamate metabolism, and Warburg effect as the main metabolic pathways. |
