A method for stabilising the XX karyotype in female mESC cultures.

Autor:	Keniry A; The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.; The Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia., Jansz N; The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.; The Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia., Hickey PF; The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.; The Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia., Breslin KA; The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia., Iminitoff M; The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.; The Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia., Beck T; The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia., Gouil Q; The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.; The Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia., Ritchie ME; The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.; The Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia., Blewitt ME; The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.; The Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2022 Nov 15; Vol. 149 (22). Date of Electronic Publication: 2022 Nov 28.
DOI:	10.1242/dev.200845
Abstrakt:	Female mouse embryonic stem cells (mESCs) present differently from male mESCs in several fundamental ways; however, complications with their in vitro culture have resulted in an under-representation of female mESCs in the literature. Recent studies show that the second X chromosome in female, and more specifically the transcriptional activity from both of these chromosomes due to absent X chromosome inactivation, sets female and male mESCs apart. To avoid this undesirable state, female mESCs in culture preferentially adopt an XO karyotype, with this adaption leading to loss of their unique properties in favour of a state that is near indistinguishable from male mESCs. If female pluripotency is to be studied effectively in this system, it is crucial that high-quality cultures of XX mESCs are available. Here, we report a method for better maintaining XX female mESCs in culture that also stabilises the male karyotype and makes study of female-specific pluripotency more feasible. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2022. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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