SOI-FET Sensors with Dielectrophoretic Concentration of Viruses and Proteins.

Autor:	Naumova O; Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Generalov V; Federal State Research Institution State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Russia.; Faculty of Automation and Computer Engineering, Novosibirsk State Technical University, 630073 Novosibirsk, Russia., Shcherbakov D; Federal State Research Institution State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Russia., Zaitseva E; Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Zhivodkov Y; Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Kozhukhov A; Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Latyshev A; Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia., Aseev A; Rzhanov Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia.; Department of Physics, Novosibirsk State University, 630090 Novosibirsk, Russia., Safatov A; Federal State Research Institution State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Russia., Buryak G; Federal State Research Institution State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Russia., Cheremiskina A; Federal State Research Institution State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Russia., Merkuleva J; Federal State Research Institution State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Russia., Rudometova N; Federal State Research Institution State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Russia.
Jazyk:	angličtina
Zdroj:	Biosensors [Biosensors (Basel)] 2022 Nov 08; Vol. 12 (11). Date of Electronic Publication: 2022 Nov 08.
DOI:	10.3390/bios12110992
Abstrakt:	Quick label-free virus screening and highly sensitive analytical tools/techniques are becoming extremely important in a pandemic. In this study, we developed a biosensing device based on the silicon nanoribbon multichannel and dielectrophoretic controlled sensors functionalized with SARS-CoV-2 spike antibodies for the use as a platform for the detection and studding of properties of viruses and their protein components. Replicatively defective viral particles based on vesicular stomatitis viruses and HIV-1 were used as carrier molecules to deliver the target SARS-CoV-2 spike S-proteins to sensory elements. It was shown that fully CMOS-compatible nanoribbon sensors have the subattomolar sensitivity and dynamic range of 4 orders. Specific interaction between S-proteins and antibodies leads to the accumulation of the negative charge on the sensor surface. Nonspecific interactions of the viral particles lead to the positive charge accumulation. It was shown that dielectrophoretic controlled sensors allow to estimate the effective charge of the single virus at the sensor surface and separate it from the charge associated with the binding of target proteins with the sensor surface.
Databáze:	MEDLINE
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