Prevalence and genetic diversity of coronaviruses, astroviruses and paramyxoviruses in wild birds in southeastern Kazakhstan.

Autor:	Zhigailov AV; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan.; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan., Maltseva ER; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan.; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan.; Tethys Scientific Society, Almaty, Kazakhstan., Perfilyeva YV; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan.; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan., Ostapchuk YO; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan.; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan., Naizabayeva DA; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan.; Al-Farabi Kazakh National University, Almaty, Kazakhstan., Berdygulova ZA; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan., Kuatbekova SA; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan., Nizkorodova AS; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan.; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan., Mashzhan A; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan.; Al-Farabi Kazakh National University, Almaty, Kazakhstan., Gavrilov AE; Institute of Zoology, Almaty, Kazakhstan., Abayev AZ; Institute of Zoology, Almaty, Kazakhstan., Akhmetollayev IA; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan., Mamadaliyev SM; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan., Skiba YA; Almaty Branch of the National Center for Biotechnology, Almaty, Kazakhstan.; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan.; Tethys Scientific Society, Almaty, Kazakhstan.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2022 Oct 31; Vol. 8 (11), pp. e11324. Date of Electronic Publication: 2022 Oct 31 (Print Publication: 2022).
DOI:	10.1016/j.heliyon.2022.e11324
Abstrakt:	Wild birds are natural reservoirs of many emerging viruses, including some zoonoses. Considering that the territory of Kazakhstan is crossed by several bird migration routes, it is important to know pathogenic viruses circulating in migratory birds in this region. Therefore, the aim of this study was to identify the host range, diversity and spatial distribution of avian paramyxoviruses, coronaviruses, and astroviruses in free-ranging wild birds in the southeastern region of Kazakhstan. For this purpose, we collected tracheal and cloacal swabs from 242 wild birds belonging to 51 species and screened them using conventional PCR assays. Overall, 4.1% (10/242) and 2.9% (7/242) of all examined birds tested positive for coronaviruses and astroviruses, respectively. Coronaviruses were found in the orders Pelecaniformes (30%; 3/10), Charadriiformes (30%; 3/10), Columbiformes (20%; 2/10), Anseriformes (10%; 1/10), and Passeriformes (10%; 1/10). All detected strains belonged to the genus Gammacoronavirus . Astroviruses were detected in birds representing the orders Passeriformes (57%; 4/7), Coraciiformes (14%; 1/7), Charadriiformes (14%; 1/7), and Columbiformes (14%; 1/7). Paramyxoviruses were observed in only two birds (0.8%; 2/242). Both strains were closely related to the species APMV-22, which had not been previously detected in Kazakhstan. Phylogenetic analysis of the partial RdRp gene sequences of the virus strains revealed three different clades of astroviruses, two clades of coronaviruses, and one clade of paramyxoviruses. The results of this study provide valuable information on the diversity and spatial distribution of paramyxoviruses, coronaviruses, and astroviruses in wild birds in southeastern Kazakhstan and highlight the importance of further thorough monitoring of wild birds in this region. Competing Interests: The authors declare no conflict of interest. (© 2022 The Author(s).)
Databáze:	MEDLINE
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