Cellular micromasonry: biofabrication with single cell precision.

Autor: Ellison ST; Department of Material Sciences and Engineering, University of Florida, Gainesville, Florida 32611, USA. t.e.angelini@ufl.edu., Duraivel S; Department of Material Sciences and Engineering, University of Florida, Gainesville, Florida 32611, USA. t.e.angelini@ufl.edu., Subramaniam V; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida 32611, USA., Hugosson F; The Whitney Laboratory for Marine Bioscience, St. Augustine, Florida 32080, USA., Yu B; Department of Chemical Engineering, University of Florida, Gainesville, Florida 32611, USA., Lebowitz JJ; Department of Neuroscience, University of Florida, Gainesville, Florida 32611, USA., Khoshbouei H; Department of Neuroscience, University of Florida, Gainesville, Florida 32611, USA., Lele TP; Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, USA.; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843, USA.; Department of Translational Medical Sciences, Texas A&M University, Houston, Texas 77843, USA., Martindale MQ; The Whitney Laboratory for Marine Bioscience, St. Augustine, Florida 32080, USA., Angelini TE; Department of Material Sciences and Engineering, University of Florida, Gainesville, Florida 32611, USA. t.e.angelini@ufl.edu.; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida 32611, USA.; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA.
Jazyk: angličtina
Zdroj: Soft matter [Soft Matter] 2022 Nov 23; Vol. 18 (45), pp. 8554-8560. Date of Electronic Publication: 2022 Nov 23.
DOI: 10.1039/d2sm01013e
Abstrakt: In many tissues, cell type varies over single-cell length-scales, creating detailed heterogeneities fundamental to physiological function. To gain understanding of the relationship between tissue function and detailed structure, and eventually to engineer structurally and physiologically accurate tissues, we need the ability to assemble 3D cellular structures having the level of detail found in living tissue. Here we introduce a method of 3D cell assembly having a level of precision finer than the single-cell scale. With this method we create detailed cellular patterns, demonstrating that cell type can be varied over the single-cell scale and showing function after their assembly.
Databáze: MEDLINE