Human Artificial Chromosomes and Their Transfer to Target Cells.
| Autor: | Ponomartsev SV; Institute of Cytology Russian Academy of Sciences, St. Petersburg, 194064 Russia., Sinenko SA; Institute of Cytology Russian Academy of Sciences, St. Petersburg, 194064 Russia., Tomilin AN; Institute of Cytology Russian Academy of Sciences, St. Petersburg, 194064 Russia.; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, 199034 Russia. |
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| Jazyk: | angličtina |
| Zdroj: | Acta naturae [Acta Naturae] 2022 Jul-Sep; Vol. 14 (3), pp. 35-45. |
| DOI: | 10.32607/actanaturae.11670 |
| Abstrakt: | Human artificial chromosomes (HACs) have been developed as genetic vectors with the capacity to carry large transgenic constructs or entire gene loci. HACs represent either truncated native chromosomes or de novo synthesized genetic constructs. The important features of HACs are their ultra-high capacity and ability to self-maintain as independent genetic elements, without integrating into host chromosomes. In this review, we discuss the development and construction methods, structural and functional features, as well as the areas of application of the main HAC types. Also, we address one of the most technically challenging and time-consuming steps in this technology - the transfer of HACs from donor to recipient cells. (Copyright ® 2022 National Research University Higher School of Economics.) |
| Databáze: | MEDLINE |
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