Morphological Characterization of Astrocytes in a Xenograft of Human iPSCDerived Neural Precursor Cells.

Autor:	Voronkov DN; Research Center of Neurology, Moscow, 125367 Russia., Stavrovskaya AV; Research Center of Neurology, Moscow, 125367 Russia., Guschina AS; Research Center of Neurology, Moscow, 125367 Russia., Olshansky AS; Research Center of Neurology, Moscow, 125367 Russia., Lebedeva OS; Federal Research and Clinical Center of Physical Chemical Medicine of the Federal Medical and Biological Agency of the Russian Federation, Moscow, 119435 Russia., Eremeev AV; Federal Research and Clinical Center of Physical Chemical Medicine of the Federal Medical and Biological Agency of the Russian Federation, Moscow, 119435 Russia., Lagarkova MA; Federal Research and Clinical Center of Physical Chemical Medicine of the Federal Medical and Biological Agency of the Russian Federation, Moscow, 119435 Russia.
Jazyk:	angličtina
Zdroj:	Acta naturae [Acta Naturae] 2022 Jul-Sep; Vol. 14 (3), pp. 100-108.
DOI:	10.32607/actanaturae.11710
Abstrakt:	Transplantation of a mixed astrocyte and neuron culture is of interest in the development of cell therapies for neurodegenerative diseases. In this case, an assessment of engraftment requires a detailed morphological characterization, in particular an analysis of the neuronal and glial populations. In the experiment performed, human iPSC-derived neural progenitors transplanted into a rat striatum produced a mixed neuron and astrocyte population in vivo by the sixth month after transplantation. The morphological characteristics and neurochemical profile of the xenografted astrocytes were similar to those of mature human astroglia. Unlike neurons, astrocytes migrated to the surrounding structures and the density and pattern of their distribution in the striatum and cerebral cortex differed, which indicates that the microenvironment affects human glia integration. The graft was characterized by the zonal features of glial cell morphology, which was a reflection of cell maturation in the central area, glial shaft formation around the transplanted neurons, and migration to the surrounding structures. (Copyright ® 2022 National Research University Higher School of Economics.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu