Microalgae as Soft Permeable Particles.

Autor: Gomes PA; Soft Matter Sciences and Engineering Laboratory, ESPCI Paris, Université PSL, Sorbonne Université, Centre National de la Recherche Scientifique, UMR 7615, 10 Rue Vauquelin, F-75005Paris, France.; Laboratoire Physico-Chimie des Interfaces Complexes, ESPCI Paris, 10 Rue Vauquelin, F-75231Paris, France.; TotalEnergies OneTech, Pôle d'Etudes et Recherche de Lacq, BP 47, 64170Lacq, France., d'Espinose de Lacaillerie JB; Soft Matter Sciences and Engineering Laboratory, ESPCI Paris, Université PSL, Sorbonne Université, Centre National de la Recherche Scientifique, UMR 7615, 10 Rue Vauquelin, F-75005Paris, France.; Laboratoire Physico-Chimie des Interfaces Complexes, ESPCI Paris, 10 Rue Vauquelin, F-75231Paris, France., Lartiges B; Géosciences Environnement Toulouse (GET), Université de Toulouse 3 (Paul Sabatier), 14 Avenue Edouard Belin, 31400Toulouse, France., Maliet M; Soft Matter Sciences and Engineering Laboratory, ESPCI Paris, Université PSL, Sorbonne Université, Centre National de la Recherche Scientifique, UMR 7615, 10 Rue Vauquelin, F-75005Paris, France., Molinier V; TotalEnergies OneTech, Pôle d'Etudes et Recherche de Lacq, BP 47, 64170Lacq, France.; Laboratoire Physico-Chimie des Interfaces Complexes, Bâtiment CHEMSTARTUP, Route Départemental 817, 64170Lacq, France., Passade-Boupat N; TotalEnergies OneTech, Pôle d'Etudes et Recherche de Lacq, BP 47, 64170Lacq, France.; Laboratoire Physico-Chimie des Interfaces Complexes, Bâtiment CHEMSTARTUP, Route Départemental 817, 64170Lacq, France., Sanson N; Soft Matter Sciences and Engineering Laboratory, ESPCI Paris, Université PSL, Sorbonne Université, Centre National de la Recherche Scientifique, UMR 7615, 10 Rue Vauquelin, F-75005Paris, France.; Laboratoire Physico-Chimie des Interfaces Complexes, ESPCI Paris, 10 Rue Vauquelin, F-75231Paris, France.
Jazyk: angličtina
Zdroj: Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2022 Nov 22; Vol. 38 (46), pp. 14044-14052. Date of Electronic Publication: 2022 Nov 07.
DOI: 10.1021/acs.langmuir.2c01735
Abstrakt: The colloidal stability of non-motile algal cells in water drives their distribution in space. An accurate description of the interfacial properties of microalgae is therefore critical to understand how microalgae concentrations can change in their biotope or during harvesting processes. Here, we probe the surface charges of three unicellular algae─ Chlorella vulgaris , Nannochloropsis oculata , and Tetraselmis suecica ─through their electrophoretic mobility. Ohshima's soft particle theory describes the electrokinetic properties of particles covered by a permeable polyelectrolyte layer, a usual case for biological particles. The results appear to fit the predictions of Ohshima's theory, proving that all three microalgae behave electrokinetically as soft particles. This allowed us to estimate two characteristic parameters of the polyelectrolyte external layer of microalgae: the volume charge density and the hydrodynamic penetration length. Results were compared with transmission electron microscopy observations of the algal cells' surfaces, and in particular of their extracellular polymeric layer, which was identified with the permeable shell evidenced by electrophoretic measurements. Noticeably, the algal surface potentials estimated from electrophoretic mobility using the soft particle theory are less negative than the apparent zeta potentials. This finding indicates that electrostatics are expected to play a minor role in phenomena of environmental and industrial importance, such as microalgae aggregation or adhesion.
Databáze: MEDLINE
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