The beneficial effects of Lacticaseibacillus casei on the small intestine and colon of Swiss mice against the deleterious effects of 5-fluorouracil.

Autor:	Barbosa SJA; Postgraduate Program in Pharmaceutical Science, Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil., Oliveira MMB; Postgraduate Program in Biotechnology /RENORBIO, Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil., Ribeiro SB; Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil., de Medeiros CACX; Postgraduate Program in Biotechnology /RENORBIO, Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil.; Postgraduate Program in Biochemistry and Molecular Biology, Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil., Lima MLS; Postgraduate Program in Oral Sciences, Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil., Guerra GCB; Postgraduate Program in Pharmaceutical Science, Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil.; Postgraduate Program in Biochemistry and Molecular Biology, Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil., de Araújo Júnior RF; Postgraduate Program in Health Sciences, Department of Morphology, Federal University of Rio Grande Norte, Natal, Brazil.; Postgraduate Program in Functional and Structural Biology UFRN, Department of Morphology, Federal University of Rio Grande Norte, Natal, Brazil., de Sousa Junior FC; Postgraduate Program in Pharmaceutical Science, Department of Pharmaceutical Science, Federal University of Rio Grande Norte, Natal, Brazil., Martins AA; Department of Dentistry, Federal University of Rio Grande Norte, Natal, Brazil., Paiva DFF; Department of Dentistry, Federal University of Rio Grande Norte, Natal, Brazil., Andrade RVS; Department of Dentistry, Federal University of Rio Grande Norte, Natal, Brazil., Rebouças CSM; Department of Morphology, Federal University of Ceará, Fortaleza, Brazil., Brito GAC; Department of Morphology, Federal University of Ceará, Fortaleza, Brazil., Leitâo RFC; Department of Morphology, Federal University of Ceará, Fortaleza, Brazil., de Araújo AA; Postgraduate Program in Pharmaceutical Science, Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil.; Postgraduate Program in Oral Sciences, Department of Biophysical and Pharmacology, Federal University of Rio Grande Norte, Natal, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2022 Oct 21; Vol. 13, pp. 954885. Date of Electronic Publication: 2022 Oct 21 (Print Publication: 2022).
DOI:	10.3389/fimmu.2022.954885
Abstrakt:	Background: Intestinal mucositis is one of the most common and important side effects of 5-fluorouracil (5-FU). Currently, there are still no specific and effective protocols for its prevention and treatment. The aim of the present study was to evaluate the effect of oral administration of Lacticaseibacillus casei ( L. casei ) on the progression of 5-FU-induced intestinal mucositis. Methods: L. casei (1x10 9 CFU/ml) or saline was orally administered to Swiss mice, beginning 15 days before intestinal mucositis induction by single intraperitoneal 5-FU administration (450 mg/kg). Body weight, number of peripheral leukocytes and fecal lactic acid bacteria were monitored. After euthanasia, on day 18, tissue samples from colon and each small intestine segment were collected for histopathology. Jejunal tissues were collected and evaluated for iNOS and TNF-alpha immunoexpression, IL-1-beta, IL-6 and TNF-alpha levels, malonaldehyde (MDA) accumulation, invertase activity and factor nuclear kappa B (NFkB-P65) gene expression, toll like receptor-4 (TLR-4), mucin-2 (MUC-2), occludin and zonula occludens-1 (ZO-1). Results: The positive impact of L. casei on 5-FU-induced leukopenia was observed, but not on 5-FU-induced weight loss in mice. L. casei reduced 5-FU-induced inflammation in the colon and small intestine (p<0.05). Decreased TNF-α, IL-1β, IL-6 (p<0.05) and MDA (p<0.05) levels, as well as decreased iNOS and TNF-alpha protein expressions (p<0.05) were found in the jejunum from L casei group. In addition, L-casei down-regulated NFKB-P65 (p<0.05) and TLR-4 (p<0.05) gene expressions and up-regulated MUC-2 and mucosal barrier proteins occludin and ZO-1 gene expressions (p<0.05). Furthermore, greater lactic acid bacteria population (p<0.05) was found in the L. casei group when compared to control groups. Conclusion: Oral L. casei administration can protect the intestine of Swiss mice from 5-FU-induced intestinal mucositis, thus contributing to overall health. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Barbosa, Oliveira, Ribeiro, de Medeiros, Lima, Guerra, de Araújo Júnior, de Sousa Junior, Martins, Paiva, Andrade, Rebouças, Brito, Leitâo and de Araújo.)
Databáze:	MEDLINE
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