Salivary microRNA profiling dysregulation in autism spectrum disorder: A pilot study.

Autor:	Kalemaj Z; UOC Maxillo-Facial Surgery and Dentistry, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, Milan, Italy., Marino MM; Department of Precision Medicine, Università della Campania 'Luigi Vanvitelli', Naples, Italy., Santini AC; Adolescents Intensive Care Unit, Centre Hospitalier Édouard Toulouse, Marseille, France., Tomaselli G; Pharmacological Research Institute Mario Negri-IRCCS, Milan, Italy.; Department of Pharmacological and Biomolecular Sciences, Università di Milano, Milan, Italy., Auti A; Department of Precision Medicine, Università della Campania 'Luigi Vanvitelli', Naples, Italy., Cagetti MG; Department of Biomedical, Surgical and Dental Science, Università di Milano, Milan, Italy., Borsello T; Pharmacological Research Institute Mario Negri-IRCCS, Milan, Italy.; Department of Pharmacological and Biomolecular Sciences, Università di Milano, Milan, Italy., Costantino A; Child and Adolescent Neuropsychiatric Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, Milan, Italy., Inchingolo F; Section of Dental Medicine, Department of Interdisciplinary Medicine, Università di Bari 'Aldo Moro', Bari, Italy., Boccellino M; Department of Precision Medicine, Università della Campania 'Luigi Vanvitelli', Naples, Italy., Di Domenico M; Department of Precision Medicine, Università della Campania 'Luigi Vanvitelli', Naples, Italy.; Department of Biology, College of Science and Technology, Temple University, Philadelphia, PA, United States., Tartaglia GM; UOC Maxillo-Facial Surgery and Dentistry, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, Milan, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in neuroscience [Front Neurosci] 2022 Oct 19; Vol. 16, pp. 945278. Date of Electronic Publication: 2022 Oct 19 (Print Publication: 2022).
DOI:	10.3389/fnins.2022.945278
Abstrakt:	Introduction: Autism spectrum disorders (ASD) are the most prevalent neurobiological disorders in children. The etiology comprises genetic, epigenetic, and environmental factors such as dysfunction of the immune system. Epigenetic mechanisms are mainly represented by DNA methylation, histone modifications, and microRNAs (miRNA). The major explored epigenetic mechanism is mediated by miRNAs which target genes known to be involved in ASD pathogenesis. Salivary poly-omic RNA measurements have been associated with ASD and are helpful to differentiate ASD endophenotypes. This study aims to comprehensively examine miRNA expression in children with ASD and to reveal potential biomarkers and possible disease mechanisms so that they can be used to improve faction between individuals by promoting more personalized therapeutic approaches. Materials and Methods: Saliva samples were collected from 10 subjects: 5 samples of children with ASD and 5 from healthy controls. miRNAs were analyzed using an Illumina Next-Generation-Sequencing (NGS) system. Results: Preliminary data highlighted the presence of 365 differentially expressed miRNAs. Pathway analysis, molecular function, biological processes, and target genes of 41 dysregulated miRNAs were assessed, of which 20 were upregulated, and 21 were downregulated in children with ASD compared to healthy controls. Conclusion: The results of this study represent preliminary but promising data, as the identified miRNA pathways could represent useful biomarkers for the early non-invasive diagnosis of ASD. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Kalemaj, Marino, Santini, Tomaselli, Auti, Cagetti, Borsello, Costantino, Inchingolo, Boccellino, Di Domenico and Tartaglia.)
Databáze:	MEDLINE
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